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Books > Science & Mathematics > Biology, life sciences > Biochemistry
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PCR Protocols (Hardcover, 3rd ed. 2011)
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PCR Protocols (Hardcover, 3rd ed. 2011)
Series: Methods in Molecular Biology, 687
Expected to ship within 10 - 15 working days
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Known for flexibility and robustness, PCR techniques continue to
improve through numerous developments, including the identification
of thermostable DNA polymerases which exhibit a range of properties
to suit given applications. PCR Protocols, Third Edition selects
recently developed tools and tricks, contributed by field-leading
authors, for the significant value that they add to more generally
established methods. Along with the cutting-edge methodologies,
this volume describes many core applications, such as PCR cloning
and sequencing, expression, copy number or methylation profile
analysis, 'DNA fingerprinting', diagnostics, protein engineering,
interaction screening as well as a chapter highlighting workflow
considerations and contamination control, crucial for all PCR
methods. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary reagents and materials,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
up-to-date, PCR Protocols, Third Edition seeks to further elucidate
this essential technique while also providing core principles with
broad applications for scientists of all backgrounds.
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