DNA delivery into cells is a rapidly developing area in gene
therapy and biotechnology. Moreover, it is a powerful research tool
to determine gene structure, regulation, and function. Viral
methods of DNA delivery are well-characterized and efficient, but
little is known about the toxicity and immunogenecity of viral
vectors. As a result, non-viral, transfection methods of DNA
delivery are of increasing interest. This comprehensive and current
resource on DNA transfection discusses the use of histidine-rich
peptides and polypeptides as DNA delivery systems and
self-assembled delivery systems based on cationic lipids and
polymers. Targeted delivery to organelles, tumour cells and
dendritic cells comprise an important topic.
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