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Books > Medicine > Clinical & internal medicine > Diseases & disorders > Immunology
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T-Cell Differentiation - Methods and Protocols (Hardcover, 1st ed. 2017)
Loot Price: R4,726
Discovery Miles 47 260
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T-Cell Differentiation - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1514
Expected to ship within 12 - 19 working days
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This volume provides protocols to successfully apply cutting-edge
technologies to characterize the biology of T cells at an
unprecedented level of complexity. Chapters guide readers through
flow cytometry and fluorescence-activated cell sorting, the
behaviour of single T cells after adoptive cell transfer (ACT),
single cell gene expression by multiplex PCR, lentiviral
transduction approaches, protocols to derive large numbers of
early-differentiated memory T cells by using dedicated cytokines
cocktails, approaches to measure telomerase activity in terminally
differentiated T cells, and approaches to define Treg cells at the
phenotypic and functional level. The final part of the book is
dedicated to the analysis of the differentiation and effector
functions of innate T cells, namely the well-known / T cells, and
the recently identified CD8+ mucosal associated invariant T (MAIT)
cells. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, T-Cell Differentiation: Methods and Protocols aims to
provide protocols that are fundamental to monitor the T cell
compartment at the level of single cells in pathological and
immunotherapy conditions.
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