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Books > Professional & Technical > Electronics & communications engineering > Electronics engineering > Applied optics > Laser technology
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Imaging, Manipulation and Optogenetics in Zebrafish (Hardcover, 1st ed. 2018)
Loot Price: R3,264
Discovery Miles 32 640
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Imaging, Manipulation and Optogenetics in Zebrafish (Hardcover, 1st ed. 2018)
Series: Springer Theses
Expected to ship within 12 - 17 working days
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Donate to Against Period Poverty
Total price: R3,284
Discovery Miles: 32 840
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The work described here investigates the advantages and limitations
of using laser light for the deep in-vivo illumination and
micromanipulation of the neuronal system in zebrafish. To do so, it
combines and develops novel optical methods such as optogenetics,
light sheet microscopy and optical micromanipulation. It also
demonstrates, for the first time, that directional and focused
laser beams can successfully be used to target large objects at
considerable depth in a living organism to exert purely optical
force - in this case on otoliths (ear stones) - and create fictive
vestibular stimuli in a stationary animal. The behavioural study
and simultaneous imaging of the whole brain reveal the location of
the brain cells specific to each ear stone. Elucidating these
fundamental neural processes holds substantial value for basic
neuroscience researchers, who still have only a vague grasp of how
brain circuits mediate perception. As such, it represents highly
innovative research that has already led to high-impact
publications and is now being intensively pursued.
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