Imaging, Manipulation and Optogenetics in Zebrafish (Hardcover, 1st ed. 2018)

The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force - in this case on otoliths (ear stones) - and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

General

Imprint:	Springer International Publishing AG
Country of origin:	Switzerland
Series:	Springer Theses
Release date:	August 2018
First published:	2018
Authors:	Itia Amandine Favre-Bulle
Dimensions:	235 x 155mm (L x W)
Format:	Hardcover
Pages:	86
Edition:	1st ed. 2018
ISBN-13:	978-3-319-96249-8
Categories:	Books > Science & Mathematics > Physics > Optics (light) Books > Science & Mathematics > Physics > Applied physics & special topics > Medical physics Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Neurosciences Books > Professional & Technical > Electronics & communications engineering > Electronics engineering > Applied optics > Laser technology
LSN:	3-319-96249-3
Barcode:	9783319962498

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