|
Books > Science & Mathematics > Biology, life sciences > Cellular biology
|
Buy Now
Cytokine Bioassays - Methods and Protocols (Hardcover, 2014 ed.)
Loot Price: R5,052
Discovery Miles 50 520
|
|
|
Cytokine Bioassays - Methods and Protocols (Hardcover, 2014 ed.)
Series: Methods in Molecular Biology, 1172
Expected to ship within 12 - 17 working days
|
Cytokines are pleiotropic regulatory proteins involved in
essentially all biological processes and associated with a wide
variety of diseases, including inflammatory disorders as well as
many types of cancer and leukemia. Knowledge about the quantitative
and qualitative nature of cytokine production is critical in the
understanding of normal and pathological processes. The cytokine
detection in biological and clinical samples faces many challenges
including their low abundance, the need to distinguish between
active and latent cytokine forms, and the need to measure multiple
cytokines in a single assay. This volume will provide a
comprehensive collection of classic and cutting-edge methodologies
that are currently used to analyze and quantify cytokines and their
biological activities in complex biological and clinical samples.
The chapters are divided into four main categories. The first group
focuses on the immunodetection of released cytokines in tissue
culture supernatants, plasma, serum, and whole blood samples by
immunoassays.These immunoassays measure the total concentrations of
released cytokines regardless of their biological activity, and
include ELISA, flow cytometry, ELISPOT, and the antibody-based
proximity ligation. . The second group will focus on the analysis
of biologically active cytokines by bioassays using neutralizing
antibodies, chemotaxis assay, cytokine-induced cell degranulation
assay, cell proliferation and differentiation, cytokine-induced
cytokine production, and the radioreceptor cytokine assay. The
third group focuses on the analysis of intracellular cytokines by
flow cytometry, western blotting and fluorescence and confocal
microscopy. In addition, this category includes protocols for
quantitative analysis of cytokine gene expression by real time
RT-PCR and analysis of the cytokine promoter occupancy by chromatin
immunoprecipitation. The fourth group focuses on the recently
developed multiplex arrays that can measure multiple cytokines in
the same sample at the same time.This group includes quantification
of multiple cytokines using cytometric bead arrays, ELISPOT assays,
proteomics cytokine evaluation, multiplexed proximity ligation
assays for high-throughput cytokine analysis, and finally, cytokine
gene expression analysis by gene arrays. The protocols will be
written by experienced basic and clinical researchers with hands-on
knowledge of the described protocols. By covering a broad variety
of methods used in cytokine detection and analysis, this book will
be of interest not only to biochemists, molecular biologists and
immunologists but also to physician-scientists working in the field
of cytokine research.
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
|
You might also like..
|
Welcome to Loot.co.za!
Sign in / Register |Wishlists & Gift Vouchers |Help | Advanced search
