Pichia Protocols focuses on recent developments of Pichia
pastoris as a recombinant protein production system. Highlighted
topics include a discussion on the use of fermentors to grow Pichia
pastoris, information on the O- and N-linked glycosylation, methods
for labeling Pichia pastoris expressed proteins for structural
studies, and the introduction of mutations in Pichia pastoris genes
by the methods of restriction enzyme-mediated integration
(REMI).
This volume fully updates and expands upon the first edition of
Pichia Protocols, and focuses primarily on information that has
come to light since its original publication. Each chapter presents
cutting-edge and cornerstone protocols for utilizing P. pastoris as
a model recomibinant protein production system.
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