In the post-genomic era, in vitro mutagenesis has emerged as a
critically important tool for establishing the functions of
components of the proteome. The third edition of In Vitro
Mutagenesis Protocols represents a practical toolbox containing
protocols vital to advancing our understanding of the connection
between nucleotide sequence and sequence function. Fully updated
from the previous editions, this volume contains a variety of
specialty tools successfully employed to unravel the intricacies of
protein-protein interaction, protein structure-function, protein
regulation of biological processes, and protein activity, as well
as a novel section on mutagenesis methods for unique microbes as a
guide to the generalization of mutagenesis strategies for a host of
microbial systems. Written in the highly successful Methods in
Molecular Biology series format, chapters include brief
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and expert tips on troubleshooting and
avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols,
Third Edition offers today's researchers a valuable compendium of
reliable and powerful techniques with which to illuminate the
proteome and its rich web of biological implications.
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