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Biotechnology - A Laboratory Course (Paperback, 2nd edition)
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Biotechnology - A Laboratory Course (Paperback, 2nd edition)
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The objectives of this Second Edition of Biotechnology: A
Laboratory Course remain unchanged: to create a text that consists
of a series of laboratory exercises that integrate molecular
biology with protein biochemistry techniques while providing a
continuum of experiments. The course begins with basic techniques
and culminates in the utilization of previously acquired technical
experience and experimental material. Two organisms, "Sacchaomyces
cerevisiae" and "Escherichia coli," a single plasmid, and a single
enzyme are the experimental material, yet the procedures and
principles demonstrated are widely applicable to other systems.
This text will serve as an excellent aid in the establishment or
instruction of introductory courses in the biological sciences.
Key Features of this new edition:
* All exercises and appendixes have been updated
* Includes new exercises on
* Polymerase chain reaction
* Beta-Galactosidase detection in yeast colonies
* Western blotting
* New procedures introduced for
* Large-scale plasmid isolation
* Yeast transformation
* DNA quantitation
* New appendixes added, one of which provides details on accessing
biological information sites on the Internet (World Wide Web)
* Use of non-radioactive materials and easy access to microbial
cultures
* Laboratory exercises student tested for seven years
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