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Yeast Genetics - A Manual of Methods (Paperback)
Loot Price: R2,909
Discovery Miles 29 090
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Yeast Genetics - A Manual of Methods (Paperback)
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The manual consists of two main sections. The first includes the
essential, sometimes laborious, procedures for handling yeasts, for
inducing mating and isolation of hybrids, for inducing sporulation
and isolation of single-spore clones, with some details of tetrad
analysis, and including techniques and ancillary equipment for use
of the micromanipulator. There are also proce dures for induction
of mutants by physical and chemical agents, and for iso lation of
particular types of mutants, such as to temperature sensitivity,
for increased frequency of mutations, for mutations in the
mitochondrial genome, both to the petite colonie form and to
resistance to antibiotics, for mutations in that part of the yeast
genome controlling the glycolytic cycle, and numerous others.
Mapping of mutations is discussed briefly, though this aspect of
yeast genetics is probably one which should not be undertaken until
the investigator has gained a certain amount of experience in the
field. How ever, as is pointed out in the pertinent part of the
manual, the task of mapping has been tremendouslysimplified by the
availability from the Yeast Genetics Stock Center at the University
of California at Berkeley of a set of auxo trophic strains designed
to permit mapping of most unknown genes with a minimum number of
crosses and tetrad analyses. The first section concludes with the
description of methods for hybridization of yeasts by protoplast
fusion, which has been described as the poor man's system for
genetic engineering."
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