Hands-on experts describe in step-by-step detail the key
methodologies of contemporary peptide synthesis and illustrate
their numerous applications. The techniques presented include
protocols for chemical ligation, the synthesis of cyclic and
phosphotyrosine-containing peptides, lipoamino acid- and
sugar-conjugated peptides, and peptide purification and analyses.
Additional chapters detail methodologies and instrumentation for
high-throughput peptide synthesis, many different applications of
peptides as novel research tools and biological probes, and the
design and application of fluorescent substrate-based peptides that
can be used to determine the selectivity and activity of
peptidases. A practical guide to the identification of proteins
using mass spectrometric analyses of peptide mixtures is also
included.
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