The first volume in this Methods Molecular Biology series, Proteins
(1984), concentrated on basic techniques for the analysis and
purification of peptides and proteins. As the series developed,
more specialized volumes on proteins were introduced, such as those
on Immunochemical Protocols (vol. 10), Practical Protein Chro-
tography (vol. 11), Analysis Glycoprotein Biomedicine (vol. 14),
Protein-DNA Interactions (vol. 30), Biomembrane Protocols (vols. 19
and 27), Analyses and Methods (vol. 17), and Optical Spectroscopy,
Microscopy, and Macroscopic Techniques (vol. 22). Further
specialist volumes on peptides, monoclonal antibodies,
immunoassays, ELISA, protein engineering, protein stability, mass
spectrometry of proteins, automated sequence analysis, and protein
NMR are currently in preparation. Since it is now a decade since
the initial volume was published, it seems an especially
appropriate moment to extensively reorganize, update, and revise
the earlier volume. In an attempt to be more c- prehensive in our
coverage, this current volume, Basic Protein and Peptide Protocols,
is totally committed to basic analytical methods; a planned
companion volume will later concentrate on preparative techniques.
Those analytical techniques requiring expensive speci- ized
instrumentation, such as NMR, mass spectrometry, X-ray cr-
tallography, spectroscopy, and automated sequence analysis, are not
described here, but in the appropriate specialized volumes listed
above.
General
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