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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Lipoprotein Protocols (Hardcover, 1998 ed.)
Loot Price: R3,052
Discovery Miles 30 520
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Lipoprotein Protocols (Hardcover, 1998 ed.)
Series: Methods in Molecular Biology, 110
Expected to ship within 10 - 15 working days
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The development of new methodologies has played a key role in the
advancement of all areas of research. Specifically, the initial
advances in our understanding of lipoprotein structure and
metabolism were made possible by the development of
ultracentrifugation and electrophoretic techniques. More recently,
the advent of molecular biological techniques opened possibilities
that were unthinkable just a few decades ago. The use of the
analytical ult- centrifuge to study plasma lipoproteins began in
the 1940s with the work of Mutzenbecher, McFarlene, Pedersen,
Gofman, Lindgren, and Elliot. Another crucial step, during the
1950s, was the development of this tool as a prepa- tive technique
by Havel, Eder, and Bragdon, among others. This technolo- cal
progress allowed investigators to "dig" deeper into the structure
of these complex macromolecules made of lipids and proteins, and
permitted inves- gators to continue unraveling the physical and
chemical characteristics of the proteins associated with
lipoprotein particles (apolipoproteins) and the enzymes involved in
their processing. This information led to both a better
understanding of the biological functions of the lipoprotein
fractions and their constituents, and creation of a more
comprehensive overall scheme for plasma lipoprotein metabolism.
Several gaps in this puzzle were filled through the work of
Goldstein and Brown, who elucidated the structure and role of the
low-density lipoprotein - ceptor. This was the first identified
among a profusion of receptors that are key for the cellular
catabolism of these particles.
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