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Innate Antiviral Immunity - Methods and Protocols (Hardcover, 1st ed. 2017)
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Innate Antiviral Immunity - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1656
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This book explores methods to study the complex and evolving
interplay between a virus and its host that range from model
systems to the detection of chemical molecules. The collection
starts with the application of humanized mice and zebrafish as
model organisms to study virus-host interactions and induction of
innate immune responses. Subsequent chapters outline diverse
methods to detect small interfering RNAs, microRNAs, and
virus-derived dsRNA from a variety of cells, tissues, and
organisms, as well as to interrogating the cytosolic RNA and DNA
sensing pathways, including using RNA PAMPs as molecular tools,
purification of cGAMP from virus particles and infected cells, and
mechanisms to visualize the subcellular localization and activation
of the adaptor proteins MAVS and STING. Cutting-edge methods,
including high-throughput and genome-wide CRISPR/Cas9 screens,
chromosome conformation capture, and whole-exome sequencing, are
described to identify novel mediators, pathways, and variants
underlying host susceptibility. Given the importance of studying
these pathways and players under physiologic conditions, methods
describing the isolation of primary mouse sensory neurons and group
2 innate lymphoid cells are also provided. Finally, this collection
comes full circle back to the whole organism level and concludes
with epidemiological methods to investigate virus-host interactions
and the induction of innate immunity. Written for the Methods in
Molecular Biology series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Innate Antiviral Immunity: Methods and Protocols spans a
diverse array of approaches to study and elucidate the intricacies
of this vital area of study. The chapter 'Morphological Separation
of Clustered Nuclei in Histological Images' is published open
access under a CC BY 4.0 license at link.springer.com.
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