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Mammary Stem Cells - Methods and Protocols (Hardcover, 2015 ed.)
Loot Price: R4,458
Discovery Miles 44 580
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Mammary Stem Cells - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1293
Expected to ship within 10 - 15 working days
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The identification of normal and breast cancer stem cells has
offered a new vision of this heterogeneous disease and new hopes
for its prognosis and treatment. This volume provides an overview
of recent developments in mammary stem cell research and discusses
the many varieties of approaches used by researchers to investigate
the properties and functions of mammary stem cells. The beginning
chapters provide readers with an introduction to mammary stem
cells, and the processes used to characterize stem cells and
isolate them via fluorescent activated cell sorting. The next few
chapters discuss DNA and mRNA sequencing, proteomic techniques to
help profile cells, lentiviral cell transduction for gene
expression, and in vivo lineage tracing. The final few chapters are
dedicated to following stem cells from their initial niche to the
new microenvironment at their metastasis site, and to studying
these cells using physical and mathematical approaches. Written in
the highly successful Methods in Molecular Biology series format,
the chapters include the kind of detailed description and
implementation advice that is crucial for getting optimal results
in the laboratory. Authoritative and cutting-edge, Mammary Stem
Cells: Methods and Protocols aims to help members of the scientific
community explore the behavior of stem cells and how to work with
them in order to guide the design of new and complimentary
strategies to be applied in the clinic with the ultimate end goal
of fighting breast cancer.
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