DNA microarray technology has revolutionized research in the
past decade. Initially an application for mRNA expression studies,
the technology now has spread to other applications such as
comparative genomic hybridization, SNP and mutation analysis. In
DNA Microarrays for Biomedical Research: Methods and Protocols,
experts explore these now commonly used applications, addressing
probe design strategies, fabrication issues and providing practical
examples of detailed methods for generation of high quality DNA
microarray data. Chapters incorporate information on some of the
largest providers of microarray, including Affymetrix, Ilumina and
Agilent, and their use on a variety of applications. Composed in
the highly successful Methods in Molecular Biology series format,
each chapter contains a brief introduction, step-by-step methods, a
list of necessary materials, and a Notes section which shares tips
on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, DNA Microarrays for
Biomedical Research: Methods and Protocols presents a variety of
protocols which can be easily reproduced, allowing researchers to
gain surprising insight into the complex world of DNA microarray
technology.
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