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Somaclonal Variation in Crop Improvement I (Hardcover, 1990 ed.)
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Somaclonal Variation in Crop Improvement I (Hardcover, 1990 ed.)
Series: Biotechnology in Agriculture and Forestry, 11
Expected to ship within 10 - 15 working days
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Genetic erosions in plant cell cultures, especially in chromosome
number and ploidy level, have now been known for over 25 years.
Until the mid -1970ssuch changes were consideredundesirable and
thereforediscarded because the main emphasis wason clonal
propagation and genetic stability of cultures. However, since the
publication on somaclonal variation by Larkin and Scowcroft (1981)
there has been a renewed interest to utilize these in vitro
obtained variations for crop improvement. Studies conduc- ted
during the last decade have shown that callus cultures, especially
on peridical subculturing over an extended period of time, undergo
morpho- logical and genetic changes, i. e. polyploidy, aneuploidy,
chromosome breakage, deletions, translocations, gene amplification,
inversions, muta- tions, etc. In addition, there are changes at the
molecular and biochemical levelsincluding changes in the DNA,
enzymes,proteins, etc. Suchchanges are now intentionally induced,
and useful variants are selected. For instance in agricultural
crops such as potato, tomato, tobacco, maize, rice and sugarcane,
plants showing tolerance to a number of diseases, viruses,
herbicides and salinity, have been isolated in cell cultures.
Likewise induction of male sterility in rice, and wheat showing
various levels of fer- tility and gliadin, have been developed in
vitro. These academic excercises open new avenues for plant
breeders and pathologists. Another area of tremendous commercial
importance in the pharmaceuti- cal industry is the selection of
cell lines showing high levels of medicinal and industrial
compounds. Already high shikonin containing somaclones in
Lithospermum are being used commercially.
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