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Protein Export and Membrane Biogenesis, Volume 4 (Hardcover)
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Protein Export and Membrane Biogenesis, Volume 4 (Hardcover)
Series: Advances in Cellular and Molecular Biology of Membranes and Organelles
Expected to ship within 12 - 17 working days
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The incentive for putting together Volume 4 of this series was to
review the wealth of new information that has become available in
prokaryotic organisms in protein export and membrane biogenesis.
Just in the last several years, protein translocation has now been
efficiently reconstituted using defined components and the
mechanism by which proteins are moved across membrane bilayers is
now being examined at a higher resolution. In addition, because of
a new technical breakthrough using osmolytes, it is now possible to
reconstitute a number of channel proteins, ATPase, receptors, and
transporters. In many cases, it is possible to successfully predict
the membrane topology of these types of proteins using both
"hydrophobicity analysis" and the "positive inside" rule.
In this volume, two chapters focus on protein translocation across
membranes ("Biochemical Analyses of Components Comprising the
Protein Translocation Machinery of E. Coli; Protein Translocation
Genetics"), while several others on how proteins assemble into the
ineer membrane of E. Coli ("Membrane Protein Assembly; Membrane
Insertion of Small Proteins: Evolutionary and Functional Aspects;
Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and
Rhodobacter Capsulatus"). Other sections review recent progress on
transporters ("Identification and Reconstitution of Anion Exchange
Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of
Lactose Permease") and signal transduction ("Mechanism of
Transmembrane Signaling in Osmoregulation") as well as the assembly
of prints into the outer membrane ("Export and Assembly of Outer
Membrane Proteins in E. coli"). Although the emphasis of the book
is on proteins, the role of phospholipids in controlling various
cell surface processes is reviewed ("Role of Phospholipids in coli
Cell Function"). I should point out the reason for the rapid
progress in bacteria research is because of the possibility to
apply biochemistry and genetics in this organism.
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