Infection by flaviviruses such as dengue virus serotypes (DENV
1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis
virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV)
impact millions of lives and cause tens of thousands of mortalities
each year. Dengue is a global public health emergency especially
since there is no preventative vaccine or antiviral treatment for
dengue disease. Dengue: Methods and Protocols offers the increasing
number of dengue researchers a one-stop protocol book with
techniques compiled from the leading laboratories working on
dengue. Chapters cover topics such as dengue virus isolation from
clinical samples, quantification of human antibodies against the
virus, assays to quantify the virus particles, the widely used
mouse model to study dengue pathogenesis, vaccine and antiviral
efficacies. Written in the successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Dengue: Methods and Protocols seeks to serve
both professionals and novices with its well-honed methodologies on
dengue research.
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