Ever since their discovery more than 150 years ago, astrocytes have
proved to be something of an enigma. Only in the last 10-15 years
has it become clear that astrocytes are multifunctional versatile
cells that play key roles in a multitude of diverse processes in
the CNS, including generation of neural stem cells, synaptogenesis,
and regulation of the blood-brain barrier and neurovascular unit.
Astrocytes: Methods and Protocols provides scientists with a
comprehensive guide to many techniques used for astrocyte cell
culture, as well as more specialized approaches for studying
astrocyte functions, both in vitro and in vivo. Key cellular,
molecular and biochemical techniques are used to study the many and
varied functions of this fascinating cell. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls.
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