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Neurotrophin Protocols (Hardcover, 2001 ed.)
Loot Price: R3,184
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Neurotrophin Protocols (Hardcover, 2001 ed.)
Series: Methods in Molecular Biology, 169
Expected to ship within 12 - 17 working days
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The past decade has seen an extraordinary growth in research
interest in neurotrophic factors, and the study of the neurotrophin
family has led this activity. Nevertheless, this area of research
has often struggled as a result of techniques that were either
inadequate or just emerging from other research fields and
disciplines. Neurotrophin Protocols has brought together many
leaders in the neurotrophin field who detail their special
expertise in a wide variety of techniques. Though most procedures
are valid across many diff- ent fields of research, some of those
described here have been developed to address particular issues
within the neurotrophic factor field. The protocols cover a broad
range of biochemical, histological, and biological techniques that
are often required by the modern laboratory. However, all have been
written with sufficient detail to allow any laboratory to achieve
proficiency without need of reference to other texts. Neurotrophin
Protocols is divided into four sections dealing with p- tein, RNA,
recombinant, and in vivo techniques. Protein techniques have in
general been less successfully employed than those dealing with RNA
or DNA. However, procedures that achieve localization and
quantification of the neurotrophins are now being used more
extensively. Their inclusion here should assist further studies at
the protein level. Transgenic cell lines and animals are
commonplace in the scientific research literature, but their inc-
sion in several chapters in this book provide some novel uses that
are not readily available elsewhere.
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