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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Recombinant Gene Expression Protocols (Hardcover)
Loot Price: R4,513
Discovery Miles 45 130
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Recombinant Gene Expression Protocols (Hardcover)
Series: Methods in Molecular Biology, 62
Expected to ship within 12 - 17 working days
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The current explosive progress in molecular biological research can
be definitively traced to the development of molecular cloning
technology. The ability to insert specific gene sequences into
cloning vectors and their subse quent expansion is the cornerstone
of modem molecular biology. A direct practical outcome of molecular
cloning technology is its application to ex press specific
recombinant genes. Currently, recombinant gene products are used in
a wide spectrum of applications, including gene therapy, production
of bioactive pharmaceuticals, synthesis of novel biopolymers, in
agriculture and animal husbandry, and so on. A fundamental
requirement for successful recombinant gene expression is the
design of the cloning vector and the choice of the host organism
for expression. Recombinant Gene Expression Protocols grows out of
the need for a laboratory manual that provides the reader the
background and rationale, as well as the practical protocols for
the preparation of "expression constructs" and their introduction
into appropriate host cells and/or organisms. The chap ters in this
book are grouped by their expression hosts, including E. coli,
yeast, mammalian cells, nonmammalian eukaryotes such as plants,
Xenopus, and insects, as well as in transgenic organisms. In-depth
information is presented on the important characteristics of
expression cloning vectors and the various methods for efficiently
introducing expression constructs into target cells and/ or
organisms. Throughout Recombinant Gene Expression Protocols, the
authors have consistently striven for a balanced presentation of
both background informa tion and actual laboratory details.
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