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Cryotechniques in Biological Electron Microscopy (Paperback, Softcover reprint of the original 1st ed. 1987)
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Cryotechniques in Biological Electron Microscopy (Paperback, Softcover reprint of the original 1st ed. 1987)
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To preserve tissue by freezing is an ancient concept going back pre
sumably to the practice of ice-age hunters. At first glance, it
seems as simple as it is attractive: the dynamics of life are
frozen in, nothing is added and nothing withdrawn except thermal
energy. Thus, the result should be more life-like than after
poisoning, tan ning and drying a living cell as we may rudely call
the conventional preparation of specimens for electron microscopy.
Countless mishaps, however, have taught electron microscopists that
cryotechniques too are neither simple nor necessarily more
life-like in their outcome. Not too long ago, experts in
cryotechniques strictly denied that a cell could truly be
vitrified, i.e. that all the solutes and macro molecules could be
fixed within non-crystalline, glass-like solid water without the
dramatic shifts and segregation effects caused by crystallization.
We now know that vitrification is indeed pos sible. Growing insight
into the fundamentals of the physics of water and ice, as well as
increasing experience of how to cool cells rapidly enough have
enlivened the interest in cryofixation and pro duced a wealth of
successful applications."
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