Contemporary approaches to the synthesis of chemically modified
biomacromolecules (proteins, nucleic acids, lipids, and
carbohydrates) not only require efficient means to control
conjugation and the specific site of attachment of the conjugated
moiety but also the effective use of recent developments in the
fields of pharmaceutical chemistry, biomolecular/polymer
engineering, and nanobiotechnology. In this second edition of
"Bioconjugation Protocols: Strategies and Methods," expert
researchers update the classic methods and introduce valuable new
approaches that go beyond basic conjugation techniques to include
elements from advanced organic synthesis, molecular biology,
surface biotechnology, materials science, and
nanobioscience/engineering. These readily reproducible methods
cover the preparation of biomolecular conjugates using a variety of
labeling techniques and semisynthetic approaches. Additional
chapters address the biofunctionalization of surface structures,
including organic/inorganic thin films, as well as various types of
nanostructures (magnetic nanoparticles, quantum dots, carbon
nanotubes, and silicon nanowire devices). All the protocols follow
the successful "Methods in Molecular Biology"TM series format, each
one offering step-by-step laboratory instructions, an introduction
outlining the principle behind the technique, lists of the
necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Cutting-edge and highly practical, "Bioconjugation Protocols:
Strategies and Methods, Second Edition" offers both novice and
experienced researchers access to the broad array of techniques
needed to carry out the semisynthesis of functional biomolecular
reagents and/or the biofunctionalization of surfaces and structures
of unique interest for a wide variety of applications, ranging from
novel biomedical diagnostics to powerful new therapeutics to
advanced biomaterials."
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