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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Neurosciences
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Neurotrophic Factors - Methods and Protocols (Hardcover, 2012 ed.)
Loot Price: R2,983
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Neurotrophic Factors - Methods and Protocols (Hardcover, 2012 ed.)
Series: Methods in Molecular Biology, 846
Expected to ship within 10 - 15 working days
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Nervous system development evolves from the well-orchestrated
processes of neural induction, cell proliferation, differentiation,
cell migration, survival, and synapse formation. Among these
environmental cues, neurotrophic factors are secreted proteins that
promote neurite outgrowth, neuronal cell differentiation and
survival both in vivo and in vitro. Nerve growth factor (NGF) is
the founding and best characterized member of the neurotrophin
family of neurotrophic polypeptides. Neurotrophic Factors: Methods
and Protocols presents a selection of protocols and procedures
which make use of cellular, tissue, and whole animal models which
can be applied to the investigation of neurotrophic factors and
other agents impacting on these systems. Chapters cover a
wide-range of topics such as dealing with the culture of neurons
and glia from the central and peripheral nervous systems,
neuron-glia co-culture models, and cell-based assays for the
evaluation of neuroprotective molecules, as well as assays which
can be applied to the study of agents with neuroregenerative
potential. Protocols describing viral- and nanoparticle-based
delivery methods to neural cells are also presented, following by
chapters dealing with organotypic slice culture protocols. Lastly,
several chapters are dedicated to in vivo lesion models of
relevance to nervous system pathology, which can be applied to the
investigation of neurotrophic factors and peptides. Written in the
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Neurotrophic
Factors: Methods and Protocols seeks to serve both professionals
and novices alike with its well-honed methodologies in an effort to
further our knowledge of what has been described as the last
frontier of science.
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