Antibody Engineering Protocols (Paperback, 1995 ed.)

Antibodies are the instraments of immune defense and attack. They can bind small atomic arrays as well as large epitopes with high affinity. Antibody Engineering Protocols presents advanced protocols in the field of antibody engineering, reviews of basic principles and methodology, and a historical perspective on the development of cur rently held beliefs about antibody structure-function relationships. The topics cover analysis of antibody sequences, three-dimensional structure, delineation of antibody characteristics in polyclonal mixtures, phage display of natural and synthetic antibodies, and antibody catalysis. Ligand recognition by antibodies occurs primarily at a subset of amino acid residues located in the complementarity determining regions (CDRs) found in the light (L) and heavy (H) chain subunits. Specific antibodies are developed by immunization with molecules identical to or related in structure to the target ligand. The immune repertoire from nonimmunized individuals also contains pre-existing specificities that can be selected by screening libraries composed of hybridoma cells or phage particles displaying F domains or indi vidual variable domains of the light (VJ and heavy (V DEGREES DEGREES) chains. Ran dom or site-directed mutagenesis in vitro can be used to refine the pre-existing specificities or produce new specificities de novo. Another level at which new specificities may be generated in vitro is V DEGREES and V DEGREES domain-swapping and CDR-swapping. The former procedure embodies a variation of natural mechanisms of generating antibody diversity. The latter procedure produces new intramolecular CDR combinations

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 51
Release date:	July 1995
First published:	1995
Editors:	Sudhir Paul
Dimensions:	235 x 155 x 23mm (L x W x T)
Format:	Paperback
Pages:	450
Edition:	1995 ed.
ISBN-13:	978-0-89603-275-0
Categories:	Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Professional & Technical > Biochemical engineering > Biotechnology > General Books > Medicine > Clinical & internal medicine > Diseases & disorders > Immunology > General Promotions
LSN:	0-89603-275-2
Barcode:	9780896032750

Is the information for this product incomplete, wrong or inappropriate? Let us know about it.

Does this product have an incorrect or missing image? Send us a new image.

Is this product missing categories? Add more categories.

Review This Product

No reviews yet - be the first to create one!