This is an overview of single molecule physics, the study of both
equilibrium and non-equilibrium properties at the single molecule
level. It begins with an introduction to this fascinating science
and includes a chapter on how to build the most popular instrument
for single molecule biophysics, the total internal reflection
fluorescence (TIRF) microscope. It concludes with the Poisson
process approach to statistical mechanics, explaining how to relate
the process to diverse areas and see how data analysis and error
bars are integral parts of science.
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