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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Handbook of Single-Cell Technologies (Mixed media product, 1st ed. 2022)
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Handbook of Single-Cell Technologies (Mixed media product, 1st ed. 2022)
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This book provides a brief overview of single-cell analysis using
recent advanced technologies. The different sections cover
different aspect of single cell analysis and applications with
their advantages, limitations, and future challenges. The book has
covered how different physical energies such as optical,
electrical, and mechanical energy have been applied for single cell
therapy and analysis. The recent advanced micro/nanofluidic devices
have been employed for single-cell counting, manipulation,
cultivation, separation, isolation, lysis, printing and patterning
and host-viral interaction at single-cell level. Various chemical
approaches for single-cell analysis have been discussed, such as
liposome mediated materials transfer at single-cell and their
analysis, discovery of antibody via single-cell, high-throughput
screening of antigen-specific antibody-secreting cells, and
biomolecular secretion analysis of individual cells. Moreover,
different single-cell omics such as genomics, proteomics and
transcriptomics have been discussed using microfluidic technologies
as well as conventional approaches. The role of single cell
analysis in system biology and biocatalysis have been discussed in
detail. The book describes single-cell phenotyping of heterogeneous
tissue, stimulation, and instant reaction quenching technology for
biochemical kinetic analysis, large scale single-cell assay for the
identification of biocatalysts and analytical techniques for
single-cell studies in microbiology. The role of single-cell
analysis in cancer, such as single-cell adhesion and cancer
progression, single-cell technologies for cancer therapy,
analytical technology for single cancer cell analysis, and
biophysical markers for cancer cell analysis have been discussed.
The flow cytometry based high throughput single-cell analysis have
been well emphasized. Finally this book has covered single-cell
electrophysiology, single-cell sensing and size measurement using
mechanical and microwave resonators, molecular force spectroscopy
for cell adhesion measurement, micro-tweezers and force microscopy
techniques for single-cell mechanobiological analysis, mass
spectrometry and acoustic tweezers for single-cell manipulation and
analysis. This book is intended for academic and industrial
researchers, undergraduate and graduate students in the fields of
biomedical engineering, bio-micro/nanoengineering, and
bio-micro/nano fabrication for single-cell analysis. It can be used
for courses on bio-MEMS/bio-NEMS, biomicrofluidics,
bio-micro/nanofabrications, micro/nanofluidics, biophysics, single
cell analysis, bionanotechnology, drug delivery systems and
biomedical microdevices. Collective contributions from respected
experts, have brought diverse aspects of single-cell technologies
in a single hand book. This will benefit researchers and
practitioners in the biotechnology industry for different diseases
analysis, therapeutics, diagnostics, drug discovery, drug screening
etc. In addition to hard copies, the book will be available online
and will often be updated by the authors.
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