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Molecular Cytogenetics - Protocols and Applications (Hardcover, 2003 ed.)
Loot Price: R4,573
Discovery Miles 45 730
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Molecular Cytogenetics - Protocols and Applications (Hardcover, 2003 ed.)
Series: Methods in Molecular Biology, 204
Expected to ship within 12 - 17 working days
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Total price: R4,593
Discovery Miles: 45 930
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The new techniques of molecular cytogenetics, mainly fluorescence
in situ hybridization (FISH) of DNA probes to metaphase chromosomes
or interphase nuclei, have been developed in the past two decades.
Many FISH techniques have been implemented for diagnostic services,
whereas some others are mainly used for investigational purposes.
Several hundreds of FISH probes and hybridization kits are now
commercially available, and the list is growing rapidly. FISH has
been widely used as a powerful diagnostic tool in many areas of
medicine including pediatrics, medical genetics, maternal-fetal
medicine, reproductive medicine, pathology, hematology, and
oncology. Frequently, a physician may be puzzled by the variety of
FISH techniques and wonder what test to order. It is not uncommon
that a sample is referred to a laboratory for FISH without
indicating a specific test. On the other hand, a cytogeneticist or
a technologist in a laboratory needs, from case to case, to
determine which procedure to perform and which probe to use for an
informative result. To obtain the best results, one must use the
right DNA probes and have reliable protocols and measures of
quality assurance in place. Also, one must have sufficient
knowledge in both traditional and molecular cytogenetics, as well
as the particular areas of medicine for which the test is used in
order to appropriately interpret the FISH results, and to correlate
them with clinical diagnosis, treatment, and prognosis.
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