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Metallomics - Recent Analytical Techniques and Applications (Hardcover, 1st ed. 2017)
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Metallomics - Recent Analytical Techniques and Applications (Hardcover, 1st ed. 2017)
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This book focuses on recent topics in metallomics, a study of the
metallome, or metal-containing biomolecules. Metals can induce
various physiological and toxicological effects in a very small
amounts, in other words, the concentrations of biometals are very
low in organisms. Thus, analytical techniques for a trace amount of
metal are crucial to understand the biological and toxicological
functions of metals.This volume begins with an overview of
metallomics including the history and development of the field.
Subsequent parts provide basic and advanced techniques for
metallomics. Speciation and imaging of metals are basic approaches
to reveal the function of the metallome. The applications of
speciation using an HPLC hyphenated with inductively coupled plasma
mass spectrometry (LC-ICP-MS) and flow cytometry ICP-MS are
described. As advanced approaches, the applications using a
micro-flow injection-ICP-MS, an ICP-triple quadrupole mass
spectrometer, an ICP-sector field mass spectrometer, and an
ICP-multi-collector mass spectrometer are mentioned. For the
imaging of metals, basic principles and applications of several
techniques such as scanning X-ray fluorescence microscopy and
ICP-MS equipped with laser ablation (LA-ICP-MS) are presented.
Speciation analyses using electrospray ionization mass spectrometry
(ESI-MS), X-ray Absorption Spectroscopy (XAS), and nuclear magnetic
resonance spectroscopy (NMR) are also introduced. The last part
highlights the medical and pharmaceutical applications of
metallomics. Molecular biological approaches to reveal the effects
of toxic metals, metal functions in brain and neurodegenerative
diseases, and metallodrugs are explained. The topic of metal
transporters is also presented.
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