This book, Medicinal and Aromatic Plants IX, like the previous eight volumes published in 1988, 1989, 1991, 1993, 1994, and 1995, is unique in its approach. It comprises twenty-four chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of Agave, Anthemis, Aralia, Blackstonia, Catha, Catharanthus, Cephalocereus, Clerodendron, Coronilla, Gloeophyllum, Liquidambar, Marchantia, Mentha, Onosma, Paeonia, Parthenium, Petunia, Phyllanthus, Populus, Portulaca, Sandersonia, Serratula, Scoparia, and Thapsia. It is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering, and plant biotechnology in general.

This series ofbooks on the biotechnology of Medicinal and Aromatic Plants provides a survey of the literature focusing on recent information and the state of the art in tissue culture and the in vitro production of secondary metabolites. This book, Medicinal and Aromatic Plants VIII, like the previous seven volumes published in 1988, 1989, 1991, 1993, and 1994, is unique in its approach. It comprises 26 chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies and the in vitro production of important medicinal and pharmaceutical compounds in various species of Achillea, Anethum, Aquilaria, Arnica, Aspergillus, Astragalus, Catalpa, Chelidonium, Eremo phila, Eucalyptus, Eucommia, Geranium, Heterocentron, Hypericum, Maclura, Morinda, Mortierella, Nicotiana, Phaseolus, Pinellia, Piqueria, Psorales, Rhodiola, Sanguisorba, Valeriana, and Vancouveria. This book is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering, and plant biotechnology in general. New Delhi, July 1995 Professor Y. P. S. BAJAJ Series Editor Contents I Achillea millefolium L. ssp. millefolium (Yarrow): In Vitro Culture and Production of Essential Oils A. C. FIGUEIREDO, M. S. S. PAIS, and J. J. c. SCHEFFER (With 9 Figures) 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 In Vitro Culture Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3 Ultrastructural Study of the Glandular Trichomes and Cell Suspension Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4 Composition of the Essential Oils of A. millefolium In Vivo and In Vitro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5 Summary and Conc1usion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 6 Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 II Anethum graveolens L."

This volume highlights achievements in cryopreservation, chronicles method development, and describes relevant literature. The provided detailed information helps practitioners develop and improve methods for desired species. The volume is divided into four parts:I. Cryopreservation of Germplasm;II. Herbaceous Plants: Barley, celery, chamomile, chicory, garlic, ginseng, hop, horseradish, mint, taro, wasabi;III. Woody Species: Coffee, Eucalyptus, guazuma, horse-chestnut, neem, olive, poplar, oak, Prunus, Ribes, rose.IV. Australian Species.Initially, cryopreservation was driven by the concern for loss of diversity of crops essential for continued improvement of the many plants used for food, health, and shelter. The interest has been expanded by conservationists and their concerns for retaining the diversity of natural populations.

Forty chapters deal with various aspects of tissue culture, in vitro manipulation, and other biotechnological approaches to the improvement of maize.
They are arranged in eight sections: - In Vitro Technology, Callus Cultures and Regeneration of Plants, Somatic Embryogenesis. - Wide Hybridization, Embryo, Ovule, and Inflorescence Culture, in Vitro Fertilization. - Production of Haploids and Double Haploids, Anther and Pollen Culture. - Protoplast Culture, Genetic Transformation. - Somaclonal Variation and Mutations. - Molecular Biology and Physiological Studies. - Proteins and Nutritional Improvement. Pollen Storage, Cryopreservation of Germplasm.

27 chapters cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture studies, and in vitro production of important medicinal and other pharmaceutical compounds in various species of Anchusa, Brucea, Catharanthus, Chrysanthemum, Coleus, Corydalis, Coreopsis, Emilia, Ginkgo, Gloriosa, Hypericum, Inonotus, Leucosceptrum, Lilium, Linum, Mosses, Nandina, Penstemon, Prunus, Pteridium, Quassia, Ribes, Senecio, Taraxacum, Thermopsis, Vanilla, and Vitiveria. Like the previous five volumes on medicinal and aromatic plants (Volumes 4, 7, 15, 21, and 24), this book contains a wealth of useful information for advanced students and researchers in the field of plant biotechnology and chemical engineering, pharmacy, botany and tissue culture.

Haploid plants have the gametophytic number of chromosomes. They are of great importance, especially in studies on the induction of muta tions and also for the production of homozygous plants, they are needed in large numbers. The conventional methods employed by plant breeders for their production are cumbersome, time-consuming, laborious and rather inefficient. Sometimes it may take years to produce a pure line. However, with the introduction of in vitro techniques, especially anther culture for the induction of androgenesis, it has become increasingly evi dent that these methods considerably accelerate the production of haploids for plant breeding programs. During the last decade, in vitro-produced haploids have been incor porated into breeding programs of many agricultural crops, and positive results have been obtained especially with rice, wheat, potato, barley, maize, asparagus, sunflower, brassica, tobacco, etc. Among these, rice and wheat are the best examples in which a number of improved varieties have been released. In wheat, the breeding cycle can be shortened by three or four generations when the pollen haploid breeding method is used instead of conventional cross-breeding. The release of the wheat varieties Jinghua 1 and Florin is a typical example of what can be achieved with other crops. Taking these developments into considera tion, the present volume, Haploids in Crop Improvement I, was compil ed."

Plant protoplasts have proved to be an excellent tool for in vitro manipulation, somatic hybridization, DNA uptake and genetic trans- formation, and for the induction of somac1onal variation. These studies reflect the far-reaching impact of protoplast research in agriculture and forest biotechnology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the literature, focusing on recent information and the state of the art in protoplast manipulation and genetic transformation. This book, Plant Protoplasts and Genetic Engineering VII, like the previous six volumes published in 1989, 1993, 1994, and 1995, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physcomi- trella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology. New Delhi, June 1996 Professor y. P. S. BAJAJ Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plantlets from Protoplasts of Allium cepa (Onion) E. E. HANSEN, J. F. HUBSTENBERGER, and G. C. PHILLIPS (With 3 Figures) 1 Introduction ...3 2 Protoplast Isolation ...4 3 Protoplast Culture ...8 4 Regeneration of Plantlets ...9 5 Summary...

Genetic erosions in plant cell cultures, especially in chromosome number and ploidy level, have now been known for over 25 years. Until the mid -1970ssuch changes were consideredundesirable and thereforediscarded because the main emphasis wason clonal propagation and genetic stability of cultures. However, since the publication on somaclonal variation by Larkin and Scowcroft (1981) there has been a renewed interest to utilize these in vitro obtained variations for crop improvement. Studies conduc- ted during the last decade have shown that callus cultures, especially on peridical subculturing over an extended period of time, undergo morpho- logical and genetic changes, i. e. polyploidy, aneuploidy, chromosome breakage, deletions, translocations, gene amplification, inversions, muta- tions, etc. In addition, there are changes at the molecular and biochemical levelsincluding changes in the DNA, enzymes,proteins, etc. Suchchanges are now intentionally induced, and useful variants are selected. For instance in agricultural crops such as potato, tomato, tobacco, maize, rice and sugarcane, plants showing tolerance to a number of diseases, viruses, herbicides and salinity, have been isolated in cell cultures. Likewise induction of male sterility in rice, and wheat showing various levels of fer- tility and gliadin, have been developed in vitro. These academic excercises open new avenues for plant breeders and pathologists. Another area of tremendous commercial importance in the pharmaceuti- cal industry is the selection of cell lines showing high levels of medicinal and industrial compounds. Already high shikonin containing somaclones in Lithospermum are being used commercially.

After the 1986 and 1989 volumes, this is the third volume on biotechnology for propagation of trees. Comprising 28 chapters contributed by international experts the book deals with fruit, ornamental, and forest trees, such as Black cherry, Sour cherry, Pomegranate, Loquat, "Ficus," Yellow poplar, Horse chestnut, Judas tree, Linden tree, Saskatoons, Taiwan sassafras, Plane-tree, Rattans, Bamboos, Engelmann spruce, White spruce, Larches, Hinoki cypress, Western redcedar, and various types of pines, i.e. Jack, Carribean, Eldarica, Slash, Egg-cone, Maritime, Ponderosa, Eastern white, Loblolly pine. Trees III is an excellent reference book for scientists, educators, and students of forestry, botany, genetics, and horticulture, who are interested in tree biotechnology.

In continuation from the previous three volumes 17, 18, and 19 on High-Tech and Micropropagation this volume presents 29 chapters on the propagation of ornamental plants through modern biotechnological methods. The species covered include "Alstromeria," "Antirrhinum," "Begonia," "Chrysanthemum," "Cornus," "Euphorbia," "Gardenia," "Gladiolus," "Hyacinthus," "Impatiens," "Iris," "Lycoris," "Nematanthus," "Paeonia," "Pelargonium," "Phalaenopsis," "Rhododendron," "Ruscus," "Saintpaulia," "Senecio," "Syringa," orchids, cacti, roses, and Boston ferns. In addition, one chapter is devoted to micropropagation of virus-free ornamentals in the CIS. Throughout the book, detailed protocols as well as a comprehensive review of the literature are provided. Advanced students, teachers, and researchers in the fields of floriculture, horticulture, and plant biotechnology in general, and also those interested in industrial or commercial micropropagation will find a wealth of useful information in the book.

Presenting the state of the art of tissue culture and in vitro propagation of vegetable and tuber crops, medicinal and aromatic plants, fibre and oilseed crops, and grasses, this book complements the previous two volumes on High-Tech and Micropropagation, which concentrated on special techniques (Vol.17) and trees and bushes of commercial value (Vol.18). The specific plants covered here include asparagus, lettuce, horse radish, cucumber, potato, cassava, sweet potato, artichoke, yams, cardamom, fennel, celery, thyme, leek, mentha, turmeric, lavender, agave, yucca, cotton, jute, sunflower, ryegrass, zoysiagrass, and various species of "Aconitum," "Artemisia," "Camelia," "Centaurium," "Digitalis," "Dioscorea," "Glehnia," "Levisticum," "Parthenium," and "Pinella." The book is of use to advanced students, teachers and research workers in the field of pharmacy, horticulture, plant breeding and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc., new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time."

'frees contribute a major part of fuel, fodder and fruit, and are an im of bioenergy. They are now needed in large numbers more portant source than ever before for afforestation and social forestry, so that fast-grow ing and multipurpose trees assume great importance. After extensive in discriminate deforestation and rapid depletion of genetic stocks, efforts are now being made to evolve methods for clonal mass propagation of improved and elite trees. Production of short-duration trees with a rapid turnover of biomass, and induction of genetic variability through in vitro manipulation for the production of novel fruit and forest trees, which are high-yielding and resistant to pests and diseases, and trees which display increased photosynthetic efficiency are in demand. These objectives are well within the realm of horticultural and forest biotech nology. Some of the recent advances, such as the regeneration of com plete trees from isolated protoplasts, somatic hybridization, and the Agrobacterium-mediated transformation in various tree species have opened new vistas for the genetic engineering of fruit and forest trees. This book is a continuation of the earlier volume Trees I, and presents 31 chapters on fruit, forest, nut and ornamental trees, such as avocado, pineapple, crabapple, quince, pistachio, walnut, hazelnut, date palm, oil palm, cacao, rubber, maple, sweet-gum, poplars, birches, Chinese tallow, willows, oaks, paper mulberry, rhododendrons, Scots pine, Calabrian pine, Douglas-fir, redwood, ginkgo, cycads and some flowering trees."

This book Trees IV, like the previous volumes (Trees I, II, III published in 1986, 1989, 1991, respectively), is special in its approach. It elucidates the case history and biotechnology of individual fruit, forest, and ornamental trees, and discusses the present state of the art, with particular reference to in vitro propagation. It comprises 24 chapters contributed by international experts, and deals with the importance, distribution, conventional propa gation, micropropagation, review of tissue culture studies, and recent advances in the in vitro culture and genetic manipulation of various species of Acrocomia, Ailanthus, Anacardium, Allocasuarina, Carya, Casuarina, Coffea, Cyphomandra, Feijoa, Fraxinus, Gymnocladus, Leptospermum, Fagus, Metroxylon, Oxydendrum, Paeonia, Paulownia, Pouteria, Psidium, and Quercus. Included are also five chapters on gymnosperm trees, such as Abies jraseri, Cephalotaxus harringtonia, Pinus durangensis, P. gregg ii, P. halepensis, P. pinea, and Tetraclinis articulata. Trees IV is a valuable reference book for scientists, teachers, and students of forestry, botany, genetics, and horticulture, and all those who are interested in the biotechnology of trees. New Delhi, March 1996 Professor y. P. S. BAJAJ Series Editor Contents Section I Angiosperm Trees 1. 1 Acrocomia Species (Macauba Palm) O. l. CROCOMO and M. MELO (With 8 Figures) 1 General Account . . . . . . . . . . 3 2 Chemical Composition . . . . . . 5 3 Genetics and Crop Improvement 9 4 In Vitro Culture Studies 10 5 Industrial Utilization . . . 14 6 Lauric Acid . . . . . . . . . 15 7 Summary and Conclusions 15 References . . . . . . . . . . . . . . . . . . . . . . . . . 16 1. 2 Ailanthus altissima Mill. Swingle (Tree of Heaven) M. ZENKTELER and B."

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of "Actinidia," "Allocasuarina," "Anthurium," "Antirrhinum," "Asparagus," "Beta," "Brassica," "Carica," "Casuarina," "Cyphomandra," "Eucalyptus," "Ipomoea," "Larix," "Limonium," "Liriodendron," "Malus," "Musa," "Physcomitrella," "Physalis," "Picea," "Rosa," "Tagetes," "Triticum," and "Ulmus."
These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

Fantasies and dreams have their rightful place in science, and sometimes they turn into reality. Regeneration of hybrid plants through protoplast fusion is one such dream come true. In the early 1970s I shared the pioneering excitement in the field of protoplast technology at the Second International Congress of Plant Tissue Culture held in Strasbourg, France. Subsequently, I participated in three international conferences devoted to plant protoplasts, in Salamanca, Spain (1972), Versailles, France (1972), and Nottingham, England (1975). At Versailles Dr. P.S. Carlson presented his work on the successful regeneration of somatic hybrids between Nicotiana glauca and Nicotiana langsdorfii. The enthusi- asm shown by the participants was sufficient indication of the bright future of somatic hybridization. On my return from Versailles, I gathered my thoughts and prepared a concept paper on Potentials of Protoplast Culture Work in Agriculture which was published in Euphytica (Bajaj 1974). The studies on protoplast fusion and somatic hybridization then gained momentum and active work started in many laboratories. Very significant work was done by Melchers et al. (1978) who obtained a somatic hybrid between potato and tomato, calling it "Pomato".

Production of food to meet the demands of an ever-increasing human population in the world is the major task and challenge to agriculture today. The conventional methods of plant breeding alone can no longer cope with the situation. The success of any crop improvement program depends on the extent of genetic variability in the base population, but due to denuding of forests and agricultural land, the naturally occurring pool of germplasm is being depleted. An urgent need is therefore ap parent to create new variability and increase the genetic base of agricul tural crops. Agricultural biotechnology has progressed to a stage in the produc tion of plants where specific characteristics to improve their yield, ap pearance, disease-resistance, nutritional quality and adaptation to ad verse soil conditions can be built into the seed. This concept of built-in quality implies a continuous scientific endeavour to improve plant char acters using a wide range of possibilities, and it also implies a scrutiny of the materials and methods available in the world today."

Plant protoplasts have proved to be an excellent tool for in vitro manipu- lations, somatic hybridization, DNA uptake and genetic transformation, and for the induction of somaclonal variation. These studies reflect the far- reaching impact of protoplast alterations for agriculture and forest bio- technology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the litera- ture, focusing on recent information and the state of the art in protoplast Plant Protoplasts manipulation and genetic transformation. This book, and Genetic Engineering VI, like the previous five volumes published in 1989,1993, and 1994, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Arachis, Bupleurum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia, Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry, pepino, peach, and plum. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general bio- technology. New Delhi, August 1995 Professor Y. P. S. BAJA] Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plants from Protop1asts of Arachis Species (Peanut) Z. LI, R. L. JARRET, and J. W. DEMSKI (With 2 Figures) 1 Introduction ...3 2 Isolation of Pro top lasts ...4 3 Culture of Protoplasts ...

Like the previous nine volumes published between 1988 and 1996, "Medicinal" "and" "Aromatic" "Plants X" is unique in its approach. It comprises 22 chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies, and the in vitro production of important medicinal and pharmaceutical compounds in various species of "Actinidia," "Alkanna," "Arnebia," "Campanula," "Catharanthus," "Centella," "Chenopodium," "Cornus," "Cynara," "Ephedra," "Euglena," "Haplophyllum," "Morus," "Oenothera," "Otacanthus," "Oxalis," "Polypodium," "Rosmarinus," "Sesamum," "Solanum," "Taxus," and "Tephrosia." This book is tailored to the needs of advanced students, teachers, and research scientists in the field of pharmacy, plant tissue culture, phytochemistry, biochemical engineering and plant biotechnology.

Somatic hybrids through the fusion of plant protoplasts have widened the genetic variability of cultivated plants. As "Somatic Hybridization in Crop Improvement I", published in 1994, this volume describes how this discipline can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization. It is divided into four sections:I. Cereals: Barley, rice, and wheat.II. Vegetables and Fruits: Arabidopsis, Asparagus, Brassica, chicory, Citrus, Cucumis, Diospyros, Ipomoea, and various Solanaceous species, e.g., tomato, potato, and eggplant.III. Medicinal and Aromatic Plants: Atropa, Dianthus, Nicotiana, and Senecio.IV. Legumes/Pasture Crops: Alfalfa.This book is tailored to the needs of advanced students, teachers and researchers in the fields of plant breeding, genetic engineering, and plant tissue culture.

Wheat, which is the second most important cereal crop in the world, is being grown in a wide range of climates over an area of about 228 945 thou sand ha with a production of about 535 842 MT in the world. Bread wheat (Triticum aestivum L. ) accounts for 80% of the wheat consumption, howe ver, it is attacked by a large number of pests and pathogens; rusts and smuts cause enormous damage to the crop and reduce the yield drastically in some areas. The major breeding objectives for wheat include grain yield, earliness, resistance to lodging and diseases, spikelet fertility, cold tolerance, leaf duration and net assimilation rate, fertilizer utilization, coleoptile length, nutritional value, organoleptic qualities, and the improvement of charac ters such as color and milling yield. The breeding of wheat by traditional methods has been practiced for centuries, however, it has only now come to a stage where these methods are insufficient to make any further breakthrough or to cope with the world's demand. Although numerous varieties are released every year around the world, they do not last long, and long-term objectives cannot be realized unless more genetic variability is generated. Moreover, the intro duction of exotic genetic stocks and their cultivation over large areas results in the depletion and loss of the native germplasm pool."

27 chapter cover the distribution, economic importance, conventional propagation, micropropagation, tissue culture, and in vitro production of important medicinal and pharmaceutical compounds in various species of Ajuga, Allium, Ambrosia, Artemisia, Aspilia, Atractylodes, Callitris, Choisya, Cinnamomum, Coluria, Cucumis, Drosera, Daucus, Eustoma, Fagopyrum, Hibiscus, Levisticum, Onobrychis, Orthosiphon, Quercus, Sanguinaria, Solanum, Sophora, Stauntonia, Tanecetum, Vetiveria, and Vitis. Like the previous volumes 4, 7, 15, and 21 in the Medicinal and Aromatic Plants series, the volume is tailored to the need of advanced students, teachers, and research scientists in the area of plant biotechnology andbioengineering, pharmacy, botany and biochemistry.

Micropropagation of plants is a multi billion dollar industry being practiced in hundreds of sm all and large nurseries and commerciallabo- ratories throughout the world. At present, it is the only component of plant biotechnology which has been commercially exploited on such a large scale, especially for the production of ornamentals. Now micropropagation of trees and medicinal plants has also assumed great importance. With recent progress made in the propagation of fruit and forest trees, and the immediate need for afforestation and planting of orchards, propagules and plantlets are required quickly and in large numbers. Taking these points into consideration High-Tech and Micropropagation I, II, III, and IV were published in 1991 and 1992. The present two volumes, High-Tech and Micropropagation V and VI, comprise 51 chapters contributed by international experts from 24 countries. High-Tech and Micropropagation V comprises 24 chapters arranged into the following three seetions: 1. Vegetables and fruits (garlic, Amaranthus, Brassica oleracea, pep- per, watermelon, cassava, banana, Myrtus communis, passionfruit, Polymnia sonchifolia, pepino, and spinaeh) H. Grasses (bamboos, Caustis dioica, Dendrocalamus, Miscanthus x giganteus, sugarcane) In. Trees (Aegle marmelos, Eucalyptus, Fraxinus excelsior, luglans cinerea, Pinus virginiana, Prosopis, and Vlmus species) High-Tech and Micropropagation VI comprises 27 chapters arranged in two sections: 1. Ornamental and aromatic plants (Amaryllis, Anthurium, Blandfordia, bromeliads, Campanula, Coleus, Ctenanthe, Cyclamen, Daphne, Dracaena, Gerbera, Helianthemum, Hippeastrum, Leucojum, Mammillaria, Maranta, Mediocactus, Mussaenda, Narcissus, Otacanthus, ponytail palm, Prunus tenella, Spiranthes, and Zinnia) II.

Micropropagation of plants is a multibillion dollar industry being prac- ticed in hundreds of small and large nurseries and commercial labora- tories throughout the world. At present, it is the only component of plant biotechnology which has been commercially exploited on such a large scale, especially for the production of ornamentals. Now micro- propagation of trees and medicinal plants has also assumed great im- portance. With recent progress made in the propagation of fruit and forest trees, and the immediate need for afforestation and planting of orchards, propagules and plantlets are required quickly and in large numbers. Taking these points into consideration High-Tech and Micro- propagation I, If, If I, and IV were published in 1991 and 1992. The present two volumes, High-Tech and Micropropagation V and VI, com- prise 51 chapters contributed by international experts from 24 countries. High-Tech and M icropropagation V comprises 24 chapters arranged into the following three sections: I. Vegetables and fruits (garlic, Amaranthus, Brassica oleracea, pepper watermelon, cassava, banana, Myrtus communis, passionfruit, Poly- mnia sonchifolia, pepino, and spinach) II. Grasses (bamboos, Caustis dioica, Dendrocalamus, Miscanthus x giganteus, sugarcane) III. Trees (Aegle marmelos, Eucalyptus, Fraxinus excelsior, Juglans cinerea, Pinus virginiana, Prosopis, and Ulmus species) High-Tech and Micropropagation VI comprises 27 chapters arranged in two sections: I.