Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging and "omics" technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways and ultimately, biological activities and functions of Ras GTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of Ras GTPases.

Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.

This book discusses the use of microorganisms for improving nutrient quality and producing healthier foods. Conventional roles of microbes in food preservation and in producing more readily digestible nutrients via natural fermentation processes are also examined. Individual chapters explore topics such as bio-preservation, incorporation of lactic acid bacteria, traditional fermented Mongolian foods, fermented fish products of Sudan, probiotics in China, fermented soymilk, food colorants, and the effect of food on gut microbiota. Readers will gain insights into current trends and future prospects of functional foods and nutraceuticals. This volume will be of particular interest to scientists working in the fields of food sciences, microbiology, agriculture and public health.

This new volume of "Methods in Enzymology" continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.
Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers hydrogen peroxide and cell signalingContains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy

This new volume of "Methods in Enzymology" continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.
Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers hydrogen peroxide and cell signalingContains chapters on such topics asthe cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling
"

First published in 1943, "Vitamins and Hormones" is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms.

Under the capable and qualified editorial leadership of Dr. Gerald Litwack, "Vitamins and Hormones" continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines.

This volume focuses on hormones and breast cancer.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

This special volume of "Progress in Molecular Biology and Translational Science "focuses on the molecular biology of arrestins, with contributions from leaders in the field. Arrestins have emerged as central players in the regulation of many facets of G protein-coupled receptor signaling. This volume covers a variety of topics with reviews written by experts in the field.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

"Advances in Botanical Research" publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 67th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on metabolomics coming of age with its technological diversity.
Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciencesFeatures a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecologyVolume features reviews on metabolomics coming of age with its technological diversity

It is widely recognized that analytical technologies and techniques are playing a pioneering role in a range of today's foremost challenging scientific endeavours, including especially biological and biomedical research. Worthy of mention, for example, are the role that high performance separation techniques played in mapping the human genome and the pioneering work done within mass spectrometry.
It is also apparent that state-of-the-art pharmaceutical and biomedical research is the major driving force of the development of new analytical techniques. Advancements in genomics research has provided the opportunity for a call for new drug targets for new technologies, which has speeded up drug discovery and helped to counteract the trend towards inflation of R&D costs.
This book has been designed to be a reference covering a wide range of protein and genomic material analysis techniques. Emerging developments are presented with applications where relevant, and biological examples are included. It was developed to meet the ever growing need for a comprehensive and balanced text on an analytical technique which has generated a tremendous amount of interest in recent years.
In addition, this book also serves as a modern textbook for advanced undergraduate and graduate courses in various disciplines including chemistry, biology and pharmacy.
Authors of the individual chapters are recognized champions of their individual research disciplines and also represent contemporary major research centres in this field.
.Contains state-of-the-art knowledge of the field and detailed descriptions of new technologies
.Provides examples of relevant applications and case studies
.Contributing authors are leading scientists in their own respective research fields"

This book presents an overview of the RNA networks controlling gene expression in fungi highlighting the remaining questions and future challenges in this area.

It covers several aspects of the RNA-mediated mechanisms that regulate gene expression in model yeasts and filamentous fungi, organisms of great importance for industry, medicine and agriculture. It is estimated that there are more than one million fungal species on the Earth. Despite their diversity (saprophytic, parasitic and mutualistic), fungi share common features distinctive from plants and animals and have been grouped taxonomically as an independent eukaryotic kingdom. In this book, 15 chapters written by experts in their fields cover the RNA-dependent processes that take place in a fungal cell ranging from formation of coding and non-coding RNAs to mRNA translation, ribosomal RNA biogenesis, gene silencing, RNA editing and epigenetic regulation.

The discovery of ribozymes nearly 30 years ago triggered a huge interest in the chemistry and biology of RNAs. Much of the recently made progress focusing on metal ions is addressed in MILS 9. This book, written by 28 internationally recognized experts from 8 nations, provides a most up-to-date view and is thus of special relevance for colleagues teaching courses in biological inorganic chemistry and for researchers dealing, e.g., with nucleic acids, gene expression, and enzymology, but also for those in analytical and bioinorganic chemistry or biophysics. Structural and Catalytic Roles of Metal Ions in RNA describes in an authoritative and timely manner in 12 stimulating chapters, supported by nearly 1600 references, 13 tables, and 75 illustrations, mostly in color, metal ion-binding motifs, methods to detect and characterize metal ion-binding sites, and the role of metal ions in folding and catalysis. It deals with diffuse metal ion binding, RNA quadruplexes, the regulation of riboswitches, metal ions and ribozymes, including artificial ribozymes. The spliceosome, the ribosome, ribozymes involving redox cofactors as well as the binding of kinetically inert metal ions to RNA are also covered.

Provides a high level reference source for scientists engaged in any aspect of plant research - chemistry, biochemistry or physiology - with primary focus on the chemistry of phosphorus-containing compounds that occur naturally in the plant kingdom, and specifically in the higher plants (Plantae).

The book is comprehensive with respect to nomenclature, physical properties, and distribution worldwide. There are many tables of actual data on phosphorus compounds occurring in whole plants and parts of plants. The tables provide detailed data that is needed by the food industry, agriculture, etc as many of the phosphorus compounds are common to both plants and animals.

Two appendices cover other aspects including changes in phosphorus-containing compounds during germination and their accumulation during growth and senescence. The final sections of the book comprise separate indexes of plants, compounds and authors.

Comprehensive examination of phosphorus compounds found in plants Extensive tables listing types of compounds and their occurrence in plants including: Nomenclature; Occurrence; Physical Properties; Synthesis; Hydrolysis; Phosphorylation; Extraction; Separation and Analysis; Easy to use indexes of plants, compounds and authors"

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

"Low-Abundance Proteome Discovery" addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authorsthat permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.
Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progressIncludes detailed protocols that address real-life needs in laboratory practiceAddresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceuticsCompiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins"

This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.

In multicellular organisms, communication between cells involves secretion of proteins that bind to receptors on neighboring cells. While this has been well documented, another mode of intercellular communication has recently become the subject of increasing interest: the release of exosomes. In cancer, tumor exosomes are involved in various aspects of pathogenesis, including proliferation, immunosuppression, and metastasis. Given the ability of exosomes to export unneeded endogenous molecules from cells, these structures hold great potential as anticancer therapeutic agents. They are also being studied as prognostic markers for cancer.

Volume 8, solely devoted to the toxicology of metals and metalloids as well as their compounds, focuses on human health. Not surprisingly, all related research areas are rapidly developing due to the role of metals and metalloids in the environment, for the work place, for food and water supply, etc. Written by 40 internationally recognized experts, the 14 stimulating chapters provide an authoritative and timely resource for scientists working in the wide range from analytical, physical, inorganic, and environmental biochemistry all the way through to toxicology, physiology, and medicine. Volume 8 highlights, supported by nearly 1900 references, in a comprehensive and timely manner the principles of risk assessment regarding the effects of metals on human health. It examines how metal ions and their compounds affect the pulmonary, cardiovascular, gastrointestinal (including liver), hematological, immune, and neurological systems, the kidney, skin and eyes, as well as human reproduction and development. MILS-8 terminates with the role of metal ions as endocrine disrupters, in genotoxicity, and cancer risk.

This contributed volume sheds new light on waste management and the production of biofuels. The authors share insights into microbial applications to meet the challenges of environmental pollution and the ever- growing need for renewable energy. They also explain how healthy and balanced ecosystems can be created and maintained using strategies ranging from oil biodegration and detoxification of azo dyes to biofouling. In addition, the book illustrates how the metabolic abilities of microorganisms can be used in microbial fuel-cell technologies or for the production of biohydrogen. It inspires young researchers and experienced scientists in the field of microbiology to explore the application of green biotechnology for bioremediation and the production of energy, which will be one of the central topics for future generations.

NMR Spectroscopy for Chemical Analysis at Low Magnetic Fields, by Stefan Gloggler, Bernhard Blumich, Stephan Appelt

Dynamic Nuclear Hyperpolarization in Liquids, by Ulrich L. Gunther

NMR with Multiple Receivers, by Eriks Kupce

TROSY NMR Spectroscopy of Large Soluble Proteins, by Yingqi Xu, Stephen Matthews

Solid-State NMR Spectroscopy of Proteins, by Henrik Muller, Manuel Etzkorn, Henrike Heise

Paramagnetic Solid-State Magic-Angle Spinning NMR Spectroscopy, by Guido Pintacuda, Gwendal Kervern"

Published continuously since 1944, the "Advances in Protein Chemistry and Structural Biology" serial has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics.

Coversreviews of methodology and research in all aspects of protein chemistry. Brings forth new information about protocols and analysis of proteins while presentingthe most recent findings from leading experts in a broad range of protein-related topics. "

This highly illustrated book provides an up-to-date description of the structure and function of the translation system including ribosomes, tRNAs, translation factors, antibiotics and aminoacyl-tRNA synthetases.

Research on translation is undergoing rapid changes and is receiving significant attention as evidenced by the Nobel Prize in Chemistry 2009. The structural research by crystallography and cryo-EM forms part of an interactive framework that involves biochemistry and molecular computation. The book provides a comprehensive overview of translation in light of the structural results. It is a valuable resource for scientists in this and related fields, as well as for students taking courses with a focus on translation.

There is no other book in this field currently except the previous edition of this book. The authors have for a long time worked in the field of structure and function of the translation system.

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.