In this book, the author provides expert analysis on naturally occurring iridoids, their chemistry and their distribution in plants and insects. Particular attention is given to the pharmacology of iridoids and their prospective applications in pharmaceutical and agricultural industries. Iridoids are found in a wide variety of plants and some insects, and they are structurally derived from monoterpenoid natural products. In the first two chapters of this book, the author describes the iridoids classification, occurrence and distribution in plants and insects. The following chapters cover different chromatographic and spectroscopic techniques that can be used to identify and quantify iridoids in herbal formulations, and also the biosynthesis of iridoids, in which the reader will discover a metabolomics and transcriptomics analysis to identify the genes involved in the biosynthesis. The final chapters provide insights on several pharmacological activities of iridoids, their physiological role in insects, pharmacokinetics in mammals, insects and microorganisms, and their applications in medicine and agriculture. This book will engage students and researchers interested in the chemistry of natural products, and it will also appeal to medicinal chemists and practitioners working in the design of new herbal drugs with bioactive pure iridoids.

Plants have developed manifold strategies and ruses for the dispersal of their seed. These are reflected in the many different colours, shapes and sizes of the fruits that contain and protect them. In this pioneering collaboration, visual artist Rob Kesseler and seed morphologist Wolfgang Stuppy use scanning electronmicroscopy to obtain astonishing images of a variety of fruits and the seeds they protect. Razor-sharp cross-sections reveal intricate interiors, nuts and other examples of botanical architecture and reproductive ingenuity. The black and white microscope images have been sumptuously coloured by Rob Kesseler highlighting the structure and functioning of the minuscule fruit and seeds some almost invisible to the naked eye and in so doing creating a work of art. Larger fruits, flowers and seeds have been especially photographed. The formation, development and demise of the fruits are described their vital role in the preservation of the biodiversity of our planet explained. Fruits are the keepers of the precious seeds that ensure our future; some are edible, others inedible and many, quite simply, incredible. Published in collaboration with Kew Royal Botanic Gardens.

This book focuses on the morphology, exine ornamentation and the associated evolutionary trends of crabapple pollen and anatomical developmental patterns. To examine the genetic evolutionary patterns of crabapple pollen traits, we constructed an interval distribution function based on characteristic pollen parameters and used a binary trivariate data matrix (Xi Yi Zi) to reflect the exine ornamentation regularity of the pollen. Our findings should inform the taxonomic status of the genus Malus. Pollen electron micrographs from a total of 26 species and 81 cultivars of Malus were recorded in this book. All 107 figures and 642 scanned pollen images constitute primary data obtained by the authors. The images in this book are clear, three-dimensional, and aesthetically pleasing. They are accompanied with text descriptions and provided a method for the indication of the different types of information that can be expected. This book can provide a reference for scientific researchers, students, and teachers in tertiary institutions that are engaged in research concerning crabapple production.

This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.

This book provides authentic and comprehensive information on the concepts, methods, functional details and applications of nano-emulsions. Following an introduction to the applications of nanotechnology in the development of foods, it elaborates on food-grade nano-emulsion and their significance, discusses various techniques and methods for producing food-grade nano-emulsion, and reviews the main ingredient and component of food-grade nano-emulsions. Further, the book includes a critical review of the engineering aspect of fabricating food-grade nano-emulsions and describe recently developed vitamin encapsulated nano-systems. In closing, it discuss the challenges and opportunities of characterizing nano-emulsified systems, the market risks and opportunities of nano-emulsified foods, and packaging techniques and safety issues - including risk identification and risk management - for nano-foods. The book offers a unique guide for scientists and researchers working in this field. It will also help researchers, policymakers, industry personnel, journalists and the general public to understand food nanotechnology in great detail.

This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Fruits of the Brazilian Cerrado: Composition and Functional Benefits describes the nutritional, chemical and physical characteristics of the fruits of the Cerrado, as well as their pharmacological effects and use in phytotherapics. Chapters are dedicated to the morphological characteristics, macronutrients, micronutrients and active compounds of various fruits, with separate sections covering their peels, leaves, nuts, pulps, and other components. The text also includes detailed studies on the treatment of diseases with these natural products, as well as their applications in popular use by local communities. Authors explain the importance of bioactive compounds found in the fruits and their possible mechanisms of action in the organism. This text thus provides a valuable reference to researchers studying a range of topics, including functional foods, phytotherapy, and plant science.

This 4-volume set focuses on the use of microbial bioremediation and phytoremediation to clean up pollutants in soil, such as pesticides, petroleum hydrocarbons, metals, and chlorinated solvents, which reduce the soil's fertility and renders it unfit for plant growth. The volumes cover the many diverse eco-friendly microbial bioremediation and phytoremediation techniques for sustainable soil management. Bioremediation and Phytoremediation Technologies in Sustainable Soil Management: Volume 1: Fundamental Aspects and Contaminated Sites begins with an overview of phytoremediation and phytotechnologies and the role of environmental factors. It goes on to introduce soil assessment techniques and offers methods of remediation designed to combat soil and agricultural degradation. Attention is given to specific types of sites and soil pollution, such as soils contaminated by heavy metals; microbial and phytoremediation-based removal of polycyclic aromatic hydrocarbons (PAHs) from coal, crude oil, and gasoline; microbial bioremediation and amelioration of pesticide-contaminated soils; phytoremediation techniques for biomedical waste contaminated sites; as well as biomediation processes for human waste sites. Biopesticides are also explained in the book as an alternative to conventional pesticides as well as the possibilities for the improvement of modern bio-pesticides. Volume 2: Microbial Approaches and Recent Trends focuses on new and emerging techniques and approaches to address soil pollution. These include the use of rhizobacteria, archae, cyanobacteria, and microalgae as biofertilizers and for soil bioremediation efforts. New technologies for assessment of soil bioremediation are explored also. The chapters provides in-depth coverage of the mechanisms, advantages, and disadvantages of the technologies used and highlights the use of different microbial enzymes that are used in the process of bioremediation and phytoremediation to clean up different pollutants without causing damage to the natural environment. Volume 3: Inventive Techniques, Research Methods, and Case Studies is organized in three themes: plants in green remediation, tools and techniques in bioremediation and phytoremediation, and special sites and their remediation techniques. Innovative new techniques that advance the use of molecular biological approaches, nanotechnology, immobilization, vermicomposting and genetic modification developments are investigated to take advantage of these possibilities. Volume 4: Degradation of Pesticides and Polychlorinated Biphenyls addresses pesticide degradation, PCBs degradation, and genetic interventions. It begins by describing environment pesticide degradation, mechanisms and sustainability, microbes and microbial enzymes, plant microbe interactions, organophosphorus degradations and endosulfan degradation. It then goes on to discuss PCBs and degradation, cypermethrin, degradation by Phanerochaete chrysosporium, carvone and surfactants for degradation of PCBs. The book also advocates for genetic systems for degradation of PCBs and pesticides, with discussion of the different advantages and disadvantages for each strategy and the various techniques. Together, these four volumes provide in-depth coverage of the mechanisms, advantages, and disadvantages of the bioremediation and phytoremediation technologies for safe and sustainable soil management. The diverse topics help to arm biologists, agricultural engineers, environmental and soil scientists and chemists with the information and tools they need to address soil toxins that are a dangerous risk to plants, wildlife, humans and, of course, the soil itself.

This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Viral Metagenomics: Methods and Protocols is a valuable resource for researchers and specialists who are interested in learning more about this evolving field.