This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutants and the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

Botanists and seed collectors Rod and Rachel Saunders set out on what seemed to some to be an impossible mission – to find and photograph, in full bloom, all gladioli species found in South Africa and to collect and share their observations in a field guide. They had found all but one species before their untimely deaths at the hands of kidnappers in a forest in KwaZulu-Natal.

In the wake of this tragic event, friends and colleagues collaborated to keep the Saunders’ dream alive. This book is the result their collective efforts.

Gladioli are showy bulbous plants that fall within the Iris family, and the bulk of species are endemic to South Africa. Comprehensive and lavishly illustrated with more than 1,300 photographs, this guide covers the 166 Gladiolus species known to occur in the region.

The book is divided into seven taxonomic sections, according to the species’ distinguishing features. Each species is presented on a double-page spread, with a full plate of color photographs, including close-ups of diagnostic features, different color forms and habitats. Botanical descriptions cover identification (corm, cataphyll, spike, bracts, anthers, pollen, capsules, seeds, and scent), distribution, pollination, conservation status, and notes on ecology and the authors’ personal observations in the field. Distribution maps and flowering periods are given for each species, and tables highlight differences between similar-looking gladioli.

There are almost 300 recognized species, widely distributed across Africa, Europe and the Middle East, with more than half of the species found in southern Africa, making this book useful to botanists world-wide, as well as to horticulturist, propagators, students, gardeners and anyone with an interest in natural history.

Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.

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The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.

Soybean genomics is of great interest as one of the most economically important crops and a major food source. This book covers recent advances in soybean genome research, including classical, RFLP, SSR, and SNP markers; genomic and cDNA libraries; functional genomics platforms; genetic and physical maps; and gene expression profiles. The book is for researchers and students in plant genetics and genomics, plant biology and pathology, agronomy, and food sciences.

This book provides an in-depth analysis of one the of most popular medicinal plants-Echinacea a species that is native to only the US and Canada. There are nine Echinacea species and several roots and above-ground portions of these showy wildflowers have been used in herbal medicine as an immune stimulant and to reduce one's chances of catching a cold. Considerable medical research supports these claims. The most popular species and the primary one wild-harvested is the one native to the Great Plains, Echinacea angustifolia. It has a long history of use, including being both historically and currently the most widely-used medicinal plant by any of the Great Plains Native Americans. The importance of this species is described by the editor with a few key contributors chosen to relate the important facets of the story of this interesting plant: Echinacea's biology, ecology, medicinal uses, markets, production and harvest, along with population biology, legal protections, ethnobotany, and history. The US Forest Service has expressed concern about the conservation status of Echinacea species on their lands, especially on the National Grasslands and National Forest units in the northern Great Plains. Overall, the future status of Echinacea, as an important medicinal plant and in the wild is not grim, but this book provides a clear perspective of why both cultivated and wild-harvested Echinacea will continue to be available to consumers without threatening the remaining populations.

In this book we present recent studies that have been carried out on some widely used medicinal plants. The need for new and alternative treatments stem from the lack of efficiency of existing remedies for certain illnesses. We have compiled information that may be useful to researchers in their quest to develop new drugs.

This is the fourth updated and revised edition of a well-received book that emphasises on fungal diversity, plant productivity and sustainability. It contains new chapters written by leading experts in the field. This book is an up-to-date overview of current progress in mycorrhiza and association with plant productivity and environmental sustainability. The result is a must hands-on guide, ideally suited for agri-biotechnology, soil biology, fungal biology including mycorrhiza and stress management, academia and researchers. The topic of this book is particularly relevant to researchers involved in mycorrhiza, especially to food security and environmental protection. Mycorrhizas are symbioses between fungi and the roots of higher plants. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition and the diversity of natural ecosystems are frequently dependent upon the pre sence and activity of mycorrhizas. The biotechnological application of mycorrhizas is expected to promote the production of food while maintaining ecologically and economically sustainable production systems.

Traditional medicinal knowledge, especially the use of ethnomedicinal plants in developing countries, has been passed down for generations. Today, however, scientists are poised to combine traditional medicinal plants and modern drug discoveries to further develop essential products that have followed the leads of indigenous cures used for centuries. Ethnomedicinal Plant Use and Practice in Traditional Medicine provides emerging research exploring the theoretical and practical aspects of indigenous knowledge and therapeutic potential within ethnobotany. Featuring coverage on a broad range of topics such as drug discovery, traditional knowledge, and herbal medicine, this book is ideally designed for doctors, healers, medical professionals, ethnobotanists, naturalists, academicians, researchers, and students interested in current research on the medical use and applications of natural-based resources.

Heavy metal accumulation in soil and water from natural sources or anthropogenic activities have produced severe environmental contamination in some parts of the world due to the persistence of metals in the environment by their accumulation throughout the food chain. The purpose of this book is to present the most recent advances in this field, mainly concerning the uptake and translocation of heavy metals in plants, mechanisms of toxicity, perception of metal and regulation of cell response under metal stress. Another key feature of this book is related to the studies on signaling and remediation processes in recent years, which have taken advantage of recent technological advances including "omic" approaches. In recent years transcriptomic, proteomic and metabolomic studies have become very important tools for analyzing both the dynamics of changes in gene expression and the profiles of protein and metabolites under heavy metal stress. This information is also very useful for plotting the complex signaling and metabolic network induced by heavy metals, in which hormones and reactive oxygen species (ROS) also play an important role. Understanding the mechanism involved in sequestration and hyperaccumulation is very important to developing new strategies of phytoremediation, which are reviewed in several chapters of this book. The information included yields very stimulating insights into the mechanism involved in the regulation of plant responses to heavy metals, which in turn improve our knowledge of cell regulation under metal stress and the use of plants for phytoremediation.

This book highlights state-of-the-art research and practices for adaptation to climate change in food production systems (agriculture in particular) as observed in Japan and neighboring Asian countries. The main topics covered include the current scientific understanding of observed and projected climate change impacts on crop production and quality, modeling of autonomous and planned adaptation, and development of early warning and/or support systems for climate-related decision-making. Drawing on concrete real-world examples, the book provides readers with an essential overview of adaptation, from research to system development to practices, taking agriculture in Asia as the example. As such, it offers a valuable asset for all researchers and policymakers whose work involves adaptation planning, climate negotiations, and/or agricultural developments.

This book highlights the implications of nanotechnology in plant sciences, particularly its potential to improve food and agricultural systems, through innovative, eco-friendly approaches, and as a result to increase plant productivity. Topics include various aspects of nanomaterials: biophysical and biochemical properties; methods of treatment, detection and quantification; methods of quantifying the uptake of nanomaterials and their translocation and accumulation in plants. In addition, the effects on plant growth and development, the role of nanoparticles in changes in gene and protein expression, and delivery of genetic materials for genetic improvement are discussed. It also explores how nanotechnology can improve plant protection and plant nutrition, and addresses concerns about using nanoparticles and their compliances. This book provides a comprehensive overview of the application potential of nanoparticles in plant science and serves as a valuable resource for students, teachers, researchers and professionals working on nanotechnology.

This edited volume provides insight into temperate fruits, with an emphasis on postharvest physiology, storage, packaging and technologies for maintaining fruit quality. Chapters are devoted to individual fruits and focus on fundamental issues such as methods for maintaining or enhancing quality, minimizing postharvest losses, and recommended technologies to boost demand. Contributions come from experts in the field, making this a key reference for all aspects of postharvest management of temperate fruits. The volume is unique in its focus on the biodiversity, nutritional and health benefits, and postharvest technologies for shelf life enhancement of temperate fruits. Contributing authors address the postharvest biology and technology of individual temperate fruits such as plum, cherry, peach, apricot, apple, pear, quince, loquat, kiwi, persimmon and berries. There has been tremendous growth in the research and development of new techniques to maintain the quality of temperate fruits from farm to table. Contributions from experts in the field cover these recent advances, providing up-to-date and relevant information for researchers, postharvest/fruit technologists, food scientists, postgraduate students, and others working in the industry.

As plants see it, humans are not the masters of the Earth but only one of its most unpleasant and irksome residents. They have been on the planet for only about 300,000 years ago (nothing compared to the three billon years of plant evolution), yet have changed the conditions of the planet so drastically as to make it a dangerous place for their own survival. It's time for the plants to offer advice. In this playful, philosophical manifesto, Stefano Mancuso, expert on plant intelligence, presents a new constitution on which to build our future as beings respectful of the Earth and its inhabitants. These eight articles - the fundamental pillars on which plant life is based - must henceforth regulate all living beings.

Drug discovery originating in Africa has the potential to provide significantly improved treatment of endemic diseases such as malaria, tuberculosis and HIV/AIDS. This book critically reviews the current status of drug discovery research and development in Africa, for diseases that are a major threat to the health of people living in Africa. Compiled by leading African and international experts, this book presents the science and strategies of modern drug discovery. It explores how the use of natural products and traditional medicines can benefit from conventional drug discovery approaches, and proposes solutions to current technological, infrastructural, human resources, and economic challenges, which are presented when attempting to engage in full-scale drug discovery. Topics addressed are varied; from African medicinal plants to marine bioprospecting, pharmacogenetics and the use of nanotechnology. This book brings together for the first time a collection of strategies and techniques that need to be considered when developing drugs in an African setting. It is an unprecedented and truly international effort, highlighting the remarkable effort made so far in the area of drug discovery research by African scientists, and scientists from other parts of the world working on African health problems.

The Rosaceae Family is represented by approximately 3,000 species of diverse plants, primarily con?ned to temperate climates. The family has a rich variety of architectural forms and contains herbaceous, tree and shrub species. Many family members are readily recognizable because of their edible seasonal fruits that are prized for their unique ?avors, colors and nutritious properties (e.g. apple, str- berry, raspberry, pear, cherry, plum, apricot, pear), as well as familiar ornamentals (e.g. roses) and nuts (e.g. almonds). Today's rosaceous cultivars have been derived from centuries of careful sel- tion and breeding, using a palette of some of evolution's most curious creations. The careful sculpting that has transformed the germplasm was not trivial, as several of the most coveted fruit species maintain complicated genomes-in some cases among the most complex of cultivated plants (e.g. strawberry). Other species in the family are represented by large perennial tree crops that exhibit substantial juven- ity phases, posing a barrier to standard breeding and genetic analyses. Yet, today's superiorcultivarsfeaturerobustgrowth,substantialyieldsandresistancetocommon biotic and abiotic stresses; traits fostered by human intervention. When the hurdles to ef?cient cultivation, breeding and selection are considered, the quality and qu- tity of rosaceous plant products derived from traditional breeding techniques is little short of amazing.

This book highlights the results from over a year of ethnobotanical research in a rural and an urban community in Jamaica, where we interviewed more than 100 people who use medicinal plants for healthcare. The goal of this research was to better understand patterns of medicinal plant knowledge, and to find out which plants are used in consensus by local people for a variety of illnesses. For this book, we selected 25 popular medicinal plant species mentioned during fieldwork. Through individual interviews, we were able to rank plants according to their frequency of mention, and categorized the medicinal uses for each species as "major" (mentioned by more than 20% of people in a community) or "minor" (mentioned by more than 5%, but less than 20% of people). Botanical identification of plant specimens collected in the wild allowed for cross-linking of common and scientific plant names. To supplement field research, we undertook a comprehensive search and review of the ethnobotanical and biomedical literature. Our book summarizes all this information in detail under specific sub-headings.

Plant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the delimitation of species, molecular approaches also provide a better understanding of evolutionary processes, a particularly important issue for some taxonomic complex groups."Molecular Plant Taxonomy: Methods and Protocols"describes laboratory protocols based on the use of nucleic acids and chromosomes for plant taxonomy, as well as guidelines for phylogenetic analysis of molecular data. Experts in the field also contribute review and application chapters that will encourage the reader to develop an integrative taxonomy approach, combining nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play.Written in the successful"Methods in Molecular Biology"series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Molecular Plant Taxonomy: Methods and Protocols"seeks to provide conceptual as well as technical guidelines to plant taxonomists and geneticists."

This volume presents a compelling collection of state-of-the-art work in algorithmic computational biology, honoring the legacy of Professor Bernard M.E. Moret in this field. Reflecting the wide-ranging influences of Prof. Moret's research, the coverage encompasses such areas as phylogenetic tree and network estimation, genome rearrangements, cancer phylogeny, species trees, divide-and-conquer strategies, and integer linear programming. Each self-contained chapter provides an introduction to a cutting-edge problem of particular computational and mathematical interest. Topics and features: addresses the challenges in developing accurate and efficient software for the NP-hard maximum likelihood phylogeny estimation problem; describes the inference of species trees, covering strategies to scale phylogeny estimation methods to large datasets, and the construction of taxonomic supertrees; discusses the inference of ultrametric distances from additive distance matrices, and the inference of ancestral genomes under genome rearrangement events; reviews different techniques for inferring evolutionary histories in cancer, from the use of chromosomal rearrangements to tumor phylogenetics approaches; examines problems in phylogenetic networks, including questions relating to discrete mathematics, and issues of statistical estimation; highlights how evolution can provide a framework within which to understand comparative and functional genomics; provides an introduction to Integer Linear Programming and its use in computational biology, including its use for solving the Traveling Salesman Problem. Offering an invaluable source of insights for computer scientists, applied mathematicians, and statisticians, this illuminating volume will also prove useful for graduate courses on computational biology and bioinformatics.

The common names of plants often cause difficulties for translators or those engaged in international studies. Although used because they are easier for non-scientists to remember than Latin or Linnean names, one species may have several common names or one common name may be used for several species. The problem is greater for weed scientists because the confusion over common names can lead to misunderstandings over control measures or the importance of weed species. The proposal to list the common names of weeds in the European languages was made in 1972 by the Joint Panel of the Evaluation of Herbicides of the European Plant Protection Organisation, and the work continued by the Working Group on Education and Training of the European Weed Research Society. The result of their labours appears in two volumes. The first is Elsevier's Dictionary of Weeds of Western Europe which was published in 1982, since when it has been a valuable source of information on the common names and importance of weed species in the countries of Western Europe. Its companion volume is this new Dictionary of Weeds of Eastern Europe. Although several books exist which give common names of plants, there are none which have the range of languages covered in these two volumes or provide information on the importance of weed species. The new dictionary will undoubtedly prove to be as welcome and as useful as its predecessor to translators, weed/crop protection scientists, botanists, ecologists, and others.

Plant-based medicines play an important role in all cultures, and have been indispensable in maintaining health and combating diseases. The identification of active principles and their molecular targets from traditional medicine provides an enormous opportunity for drug development. Using modern biotechnology, plants with specific chemical compositions can be mass propagated and genetically improved for the extraction of bulk active pharmaceuticals. Although there has been significant progress in the use of biotechnology, using tissue cultures and genetic transformation to investigate and alter pathways for the biosynthesis of target metabolites, there are many challenges involved in bringing plants from the laboratory to successful commercial cultivation. This book presents the latest advances in the development of medicinal drugs, including topics such as plant tissue cultures, secondary metabolite production, metabolomics, metabolic engineering, bioinformatics and future biotechnological directions.