Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape. These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.

Plants possess the ability to biosynthesize a large variety of steroids, but it was not until 1979 that a hormonal function was demonstrated in plants. Today, about 40 structurally and functionally related steroids, known as brassinosteroids, have been isolated from natural sources. Brassinosteroids demonstrate various kinds of regulatory activities in the growth and development of plants. This book is based on a 1990 Russian monograph, but includes all important subsequent literature and developments, including unpublished data from the authors' laboratories.
Key Features
* BRASSINOSTEROIDS: A New Class of Plant Hormones covers:
* Structures and classification
* Isolation and spectroscopic determination
* Biosynthesis and metabolism
* Natural product synthesis
* Physiological mode of action
* Structure-activity relationships
* Practical applications in agriculture

Plants form mutualistic association with various microorganisms, particularly in the rhizosphere region. The association benefits both the partners in a number of ways. A single plant can support the growth of diverse microbes and in reciprocation these microbes help the plant in several ways. A great deal of knowledge is now available on the mechanisms of action of plant growth promoting microbes in forming association with their partner plant and benefitting it. With ever increasing population and to achieve food security it has become utmost necessary to utilize these friendly microbes to enhance the crop yield and quality in an ecofriendly and sustainable manner. We already know about the huge negative impact of chemicals used in agriculture on the humans and the ecosystems as whole. 'Plant Microbes Symbiosis - Applied Facets' provides a comprehensive knowledge on practical, functional and purposeful utility of plant-microbe interactions. The book reviews the utilization of beneficial microbes for crop yield enhancement and protection against diseases caused by phytopathogens and nutrient deficiencies. The tome also reviews the utility of plant growth promoting microbes in helping the plants to deal with abiotic stresses imposed by climate change and anthropogenic activities. The book showcases how plant-microbe interactions are or can be utilized for reclamation of stressed soils and degradation of pollutants in a most effective and environment friendly manner. It also ascertains the reasons for the below par performance of the microbial based inoculants. The utilization of biotechnological tools for development of next generation bioformulations to combat the new challenges and overcome past hurdles has been discussed. This wonderful association between plants and microbes if used properly will not only enhance the crop yields and reclaim barren lands but also make our planet a better place to live on for all of its habitants.

The first synthesis of current research regarding Everglades microbial community structure and function, this book provides an understanding of the physical and chemical factors affecting the structure of microbial communities, including nutrient effects, sea level rise, and other potential stressors. The book integrates traditional research on algal and bacterial structure and function, helping to provide a more holistic understanding of the varying microbial communities throughout the Everglades. From periphyton, to soils and detritus, to flocculent organic matter, Microbiology of the Everglades Ecosystem covers new and emerging methods and their global application.

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book's main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology (TM) series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

The field of redox is rapidly changing, specifically in relation toplants whereredox reactions are exacerbated compared to non-photosynthetic organisms. The development of proteomics has allowed the identification of hundreds of molecular targets of these systems, andthe recent discovery of glutaredoxin's ability to bind iron sulfur centers (ISCs) and to participate in ISC assembly in other apoproteins has provided many new insights. This volume presents new research on oxidative stress in plants, ranging from the production of reactive oxygen species or reactive nitrogen species, to their accumulation, their involvement in signal transduction, and their degradation, while also covering the links among oxidative stress and biotic and abiotic stresses.

* Cutting-edge reviews written from a broad range of scientific perspectives * For over 40 years, series has enjoyed a reputation for excellence * Contributors internationally recognized authorities in their respective fields"

"Plant and Process Engineering 360" will be the backbone of any plant, chemical, or process engineer s library. This is a broad area in which engineers need to be familiar with a wide array of techniques, technologies andequipment. Its focus on providing a broad introduction to key systems make the book the first point of reference for engineers who are involved with designing, specifying, maintaining or working with plant, process and control technologies in many sectors, includingmanufacturing, chemical process, and energy.
A single-source of plant and processequipment information for engineers, providinga 360 degree view of the critical equipment engineers encounterEnablesreaders to get up to speed with unfamiliar topics quickly withan overview of important but disparate technologies that are specific to plant engineering Coversthe systems and processes that drive effective and efficient plants and processesDrawn from authoritative Elsevier resources, this book is a first port of call with breadth and depth of content, from leading figures in the field."

This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.

This book summarizes the taxonomic details of selected fossil angiosperm pollen genera and species along with their affinity and occurrences in space and time. It provides information about the historical background of palynology from Palaeogene and Neogene sediments in India, general pollen morphology along with the terms commonly used in describing fossil angiosperm pollen and an overview of the Indian Tertiary sediments. A complete list of genera described in this book is given. It also includes a key for identification of Indian angiosperm genera as well as a description of selected fossil angiosperm pollen from India along with their Indian records, illustrations, locality, age and horizon from where these have been reported. In all 130 genera and 402 species have been dealt with. The names of genera and species are arranged alphabetically. The Discussion part includes a summarized account of palynofloras, their biostratigraphic application in various regions of India and their palaeogeographical and palaeoclimatic implications.

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.

Have you ever wondered why the leaves of the Swiss cheese plant have holes? How aloe vera came to be harnessed as a medicinal powerhouse? Or why – despite your best efforts – you can’t keep your Venus flytrap alive? You are not alone: houseplant expert Jane Perrone has asked herself those very questions, and in Legends of the Leaf she digs deep beneath the surface to reveal the answers. By exploring how they grow in the wild, and the ways they are understood and used by the people who live among them, we can learn almost everything we need to know about our cherished houseplants. Along the way, she unearths their hidden histories and the journeys they’ve taken to become prized possessions in our homes: from the Kentia palms which stood either side of Queen Victoria’s coffin as she lay in state; to the dark history of the leopard lily, once exploited for its toxic properties; to English ivy, which provided fishermen with a source of bait. Each houseplant history in this beautifully illustrated collection is accompanied by a detailed care guide and hard-won practical advice, but it is only by understanding their roots that we can truly unlock the secrets to helping plants thrive.

Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.

"

The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use, Volume One, Third Edition has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community (new mutant libraries, new omics studies, and potentially more information about different Chlamydomonas species in the environment). In addition to updates on molecular techniques and analysis of the sequenced genome, the book presents the latest in research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, and more.

This edited book details the plant-assisted remediation methods, which involves the interaction of plant roots with associated rhizospheric microorganisms for the remediation of soil and water contaminated with high levels of heavy metals, pesticides, radionuclides, agricultural by-products, municipal wastes, industrial solvents, petroleum hydrocarbons, organic compounds, and various other contaminants. Each chapter highlights and compares the beneficial and economical alternatives of phytoremediation to currently practiced soil, water, and air removal. This book covers state-of-the-art approaches in phytoremediation contributed by leading and eminent scientists from across the world. Phytoremediation approaches for environmental sustainability dealing the readers with a cutting-edge of multidisciplinary understanding in the principal and practical approaches of phytoremediation from laboratory research to field application. This book is of interest to researchers, teachers, environmental scientists, environmental engineers, environmentalists, and policy makers. Also, the book serves as additional reading material for undergraduate and graduate students of environmental microbiology, biotechnology, eco-toxicology, environmental remediation, waste management, and environmental sciences as well as the general audience.

Soybean genomics is of great interest as one of the most economically important crops and a major food source. This book covers recent advances in soybean genome research, including classical, RFLP, SSR, and SNP markers; genomic and cDNA libraries; functional genomics platforms; genetic and physical maps; and gene expression profiles. The book is for researchers and students in plant genetics and genomics, plant biology and pathology, agronomy, and food sciences.

This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutants and the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

Edited by Jean-Claude Kader and Michel Delseny and supported by an international Editorial Board, "Advances in Botanical Research" publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 50th volume, the series features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This eclectic volume features six reviews on cutting-edge topics of interest to postgraduates and researchers alike.
* Multidisciplinary reviews written from a broad range of scientific perspectives
* For over 40 years, series has enjoyed a reputation for excellence
* Contributors internationally recognized authorities in their respective fields

The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.

This is the first English-language book dedicated to Brazilian sand flies and their medical importance. No other country has so many species of these haematophagous insects as Brazil and their diversity has reached an astonishing level. The book contains comprehensive chapters, written by Brazilian experts on their regional distribution, their ecology and their importance as vectors of pathogens and parasites. Methods for sampling, processing and preserving phlebotomines are reviewed as are perspectives on surveillance and leishmaniasis vector control. A novel classification is presented whose aim is to help investigators identify the species that they are working with more efficiently.

This book provides an in-depth analysis of one the of most popular medicinal plants-Echinacea a species that is native to only the US and Canada. There are nine Echinacea species and several roots and above-ground portions of these showy wildflowers have been used in herbal medicine as an immune stimulant and to reduce one's chances of catching a cold. Considerable medical research supports these claims. The most popular species and the primary one wild-harvested is the one native to the Great Plains, Echinacea angustifolia. It has a long history of use, including being both historically and currently the most widely-used medicinal plant by any of the Great Plains Native Americans. The importance of this species is described by the editor with a few key contributors chosen to relate the important facets of the story of this interesting plant: Echinacea's biology, ecology, medicinal uses, markets, production and harvest, along with population biology, legal protections, ethnobotany, and history. The US Forest Service has expressed concern about the conservation status of Echinacea species on their lands, especially on the National Grasslands and National Forest units in the northern Great Plains. Overall, the future status of Echinacea, as an important medicinal plant and in the wild is not grim, but this book provides a clear perspective of why both cultivated and wild-harvested Echinacea will continue to be available to consumers without threatening the remaining populations.

"Comparative Plant Virology" provides a complete overview of our current knowledge of plant viruses, including background information on plant viruses and up-to-date aspects of virus biology and control. It deals mainly with concepts rather than detail. The focus will be on plant viruses but due to the changing environment of how virology is taught, comparisons will be drawn with viruses of other kingdomes, animals, fungi and bacteria. It has been written for students of plant virology, plant pathology, virology and microbiology who have no previous knowledge of plant viruses or of virology in general.
* Boxes highlight important information such as virus definition and taxonomy
* Includes profiles of 32 plant viruses that feature extensively in the text
* Companion website providing image bank
* Full color throughout