In this book, the author provides expert analysis on naturally occurring iridoids, their chemistry and their distribution in plants and insects. Particular attention is given to the pharmacology of iridoids and their prospective applications in pharmaceutical and agricultural industries. Iridoids are found in a wide variety of plants and some insects, and they are structurally derived from monoterpenoid natural products. In the first two chapters of this book, the author describes the iridoids classification, occurrence and distribution in plants and insects. The following chapters cover different chromatographic and spectroscopic techniques that can be used to identify and quantify iridoids in herbal formulations, and also the biosynthesis of iridoids, in which the reader will discover a metabolomics and transcriptomics analysis to identify the genes involved in the biosynthesis. The final chapters provide insights on several pharmacological activities of iridoids, their physiological role in insects, pharmacokinetics in mammals, insects and microorganisms, and their applications in medicine and agriculture. This book will engage students and researchers interested in the chemistry of natural products, and it will also appeal to medicinal chemists and practitioners working in the design of new herbal drugs with bioactive pure iridoids.

This 4-volume set focuses on the use of microbial bioremediation and phytoremediation to clean up pollutants in soil, such as pesticides, petroleum hydrocarbons, metals, and chlorinated solvents, which reduce the soil's fertility and renders it unfit for plant growth. The volumes cover the many diverse eco-friendly microbial bioremediation and phytoremediation techniques for sustainable soil management. Bioremediation and Phytoremediation Technologies in Sustainable Soil Management: Volume 1: Fundamental Aspects and Contaminated Sites begins with an overview of phytoremediation and phytotechnologies and the role of environmental factors. It goes on to introduce soil assessment techniques and offers methods of remediation designed to combat soil and agricultural degradation. Attention is given to specific types of sites and soil pollution, such as soils contaminated by heavy metals; microbial and phytoremediation-based removal of polycyclic aromatic hydrocarbons (PAHs) from coal, crude oil, and gasoline; microbial bioremediation and amelioration of pesticide-contaminated soils; phytoremediation techniques for biomedical waste contaminated sites; as well as biomediation processes for human waste sites. Biopesticides are also explained in the book as an alternative to conventional pesticides as well as the possibilities for the improvement of modern bio-pesticides. Volume 2: Microbial Approaches and Recent Trends focuses on new and emerging techniques and approaches to address soil pollution. These include the use of rhizobacteria, archae, cyanobacteria, and microalgae as biofertilizers and for soil bioremediation efforts. New technologies for assessment of soil bioremediation are explored also. The chapters provides in-depth coverage of the mechanisms, advantages, and disadvantages of the technologies used and highlights the use of different microbial enzymes that are used in the process of bioremediation and phytoremediation to clean up different pollutants without causing damage to the natural environment. Volume 3: Inventive Techniques, Research Methods, and Case Studies is organized in three themes: plants in green remediation, tools and techniques in bioremediation and phytoremediation, and special sites and their remediation techniques. Innovative new techniques that advance the use of molecular biological approaches, nanotechnology, immobilization, vermicomposting and genetic modification developments are investigated to take advantage of these possibilities. Volume 4: Degradation of Pesticides and Polychlorinated Biphenyls addresses pesticide degradation, PCBs degradation, and genetic interventions. It begins by describing environment pesticide degradation, mechanisms and sustainability, microbes and microbial enzymes, plant microbe interactions, organophosphorus degradations and endosulfan degradation. It then goes on to discuss PCBs and degradation, cypermethrin, degradation by Phanerochaete chrysosporium, carvone and surfactants for degradation of PCBs. The book also advocates for genetic systems for degradation of PCBs and pesticides, with discussion of the different advantages and disadvantages for each strategy and the various techniques. Together, these four volumes provide in-depth coverage of the mechanisms, advantages, and disadvantages of the bioremediation and phytoremediation technologies for safe and sustainable soil management. The diverse topics help to arm biologists, agricultural engineers, environmental and soil scientists and chemists with the information and tools they need to address soil toxins that are a dangerous risk to plants, wildlife, humans and, of course, the soil itself.

Bioassays: Advanced Methods and Applications provides a thorough understanding of the applications of bioassays in monitoring toxicity in aquatic ecosystems. It reviews the newest tests and applications in discovering compounds and toxins in the environment, covering all suitable organisms, from bacteria, to microorganisms, to higher plants, including invertebrates and vertebrates. By learning about newer tests, water pollution control testing can be less time and labor consuming, and less expensive. This book will be helpful for anyone working in aquatic environments or those who need an introduction to ecotoxicology or bioassays, from investigators, to technicians and students.

This manual brings together information on all phases of seed handling and presents the results of more than 20 years of studies. Forest Service field personnel at several experiment stations and regional offices furnished a backlog of source material for treatments of individual genera. The manual consists of two main parts. Part 1 formulates general principles on the various phases of seed handling from formation of the seed to sowing. Part 2, which forms the larger part of the manual, provides relatively detailed but concise information for 444 species and varieties of trees and shrubs; this includes data on distribution and use, discussions of seeding habits, methods of seed collection, extraction and storage, seed germination, and nursery and field practice.

This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This book provides authentic and comprehensive information on the concepts, methods, functional details and applications of nano-emulsions. Following an introduction to the applications of nanotechnology in the development of foods, it elaborates on food-grade nano-emulsion and their significance, discusses various techniques and methods for producing food-grade nano-emulsion, and reviews the main ingredient and component of food-grade nano-emulsions. Further, the book includes a critical review of the engineering aspect of fabricating food-grade nano-emulsions and describe recently developed vitamin encapsulated nano-systems. In closing, it discuss the challenges and opportunities of characterizing nano-emulsified systems, the market risks and opportunities of nano-emulsified foods, and packaging techniques and safety issues - including risk identification and risk management - for nano-foods. The book offers a unique guide for scientists and researchers working in this field. It will also help researchers, policymakers, industry personnel, journalists and the general public to understand food nanotechnology in great detail.

This book covers the applications of fungi used in biorefinery technology. As a great many different varieties of fungal species are available, the text focuses on the various applications of fungi for production of useful products including organic acids (lactic, citric, fumaric); hydrolytic enzymes (amylase, cellulases, xylanases, ligninases, lipases, pectinases, proteases); advanced biofuels (ethanol, single cell oils); polyols (xylitol); single cell protein (animal feed); secondary metabolites; and much more.

With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.