This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Senescence: Methods and Protocols aims to provide a useful hand book of standard protocols for plant molecular biologists working on senescence.

In these short illustrated guides, Dr Mark Everard, avid nature-watcher, angler and scientist, takes a dedicated look at three British freshwater fishes, the Silver Bream, Gudgeon and Ruffe. Though an integral part of aquatic ecosystems and well-known to anglers, these fish are often overlooked by the wider public as well as scientists. Each book is in three sections, first exploring the biology of the fish itself, including science written in accessible style, second discussing angling history and tips, and thirdly exploring the fish's cultural connections, including etymology of the fish. A bibliography at the end of each guide directs the reader to additional resources.

This volume presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of Brachypodium that will help researchers better understand the model plant using NGS technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Brachypodium Genomics: Methods and Protocols is a valuable resource for bench-oriented molecular biologists and computational biologists working towards further evolving this field.

This volume presents a comprehensive perspective of the biopesticides Bacillus thuringiensis and Lysinibacillus sphaericus, from their basic biology to agriculture, forestry and public-health applications. It covers their ecology, virulence factors, and genetic characterization. The topics related to agriculture and forestry include mode of action, receptors of insect pests, and heterologous expression of toxins in insect cells and plants. Public-health researchers will find information on vector control programs with an emphasis on the Neotropical region. The book also discusses new products and the global market.

This book is a compilation of information on insect/mite/vertebrate pests and fungal/bacterial/viral/mycoplasma/nematode diseases of tropical root and tuber crops such as cassava, sweet potato, yams, taro, Amorphophallus, yam bean and tannia. The book highlights the distribution, symptoms and damage, biology, survival and spread of each pest and describes management methods. It also sheds light on different eco-friendly pest management strategies including physical, cultural, chemical, biological, host resistance and integrated methods. The book is written in a lucid style using easy-to-understand language and offers adoptable recommendations involving eco-friendly control measures. It serves as a useful reference source for policy makers, research and extension workers, practicing farmers and students. The material can also be used for teaching post graduate courses in state agricultural universities.

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

An introduction to the principles of membrane transport: How molecules and ions move across the cell membrane by simple diffusion and by making use of specialized membrane components (channels, carriers, and pumps). The text emphasizes the quantitative aspects of such movement and its interpretation in terms of transport kinetics. Molecular studies of channels, carriers, and pumps are described in detail as well as structural principles and the fundamental similarities between the various transporters and their evolutionary interrelationships. The regulation of transporters and their role in health and disease are also considered.

This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.

This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.

This two-volume book is an up-to-date revision of methods and principles of phylogenetic analysis of morphological data. It is also a general guide for using the computer program TNT in the analysis of such data. The book covers the main aspects of phylogenetic analysis, and general methods to compare classifications derived from molecules and morphology. Practical application of all principles discussed is illustrated by reference to TNT, a freely available software package that can perform all the steps needed in a phylogenetic analysis. Key Features Provides in depth discussion of the connections between hypotheses of homology and the parsimony criterion Helps understand the basis for designing different aspects of a morphological study, from hints on delimiting the problem and data collecting, to character definitions and analysis Discusses the applicability of statistical models coopted from sequence analysis to the analysis of morphological data Gives detailed descriptions of the principles behind algorithms for evaluating and searching phylogenetic trees Presents extensive information on commands and options of the TNT computer program

Abiotic stresses such as drought (water deficit), extreme temperatures (cold, frost and heat), salinity (sodicity) and mineral (metal and metalloid) toxicity limit productivity of crop plants worldwide and are big threats to global food security. With worsening climate change scenarios, these stresses will further increase in intensity and frequency. Improving tolerance to abiotic stresses, therefore, has become a major objective in crop breeding programs. A lot of research has been conducted on the regulatory mechanisms, signaling pathways governing these abiotic stresses, and cross talk among them in various model and non-model species. Also, various 'omics' platforms have been utilized to unravel the candidate genes underpinning various abiotic stresses, which have increased our understanding of the tolerance mechanisms at structural, physiological, transcriptional and molecular level. Further, a wealth of information has been generated on the role of chromatin assembly and its remodeling under stress and on the epigenetic dynamics via histones modifications. The book consolidates outlooks, perspectives and updates on the research conducted by scientists in the abovementioned areas. The information covered in this book will therefore interest workers in all areas of plant sciences. The results presented on multiple crops will be useful to scientists in building strategies to counter these stresses in plants. In addition, students who are beginners in the areas of abiotic stress tolerance will find this book handy to clear their concepts and to get an update on the research conducted in various crops at one place

The instant New York Times bestseller | A Washington Post Notable Book | One of NPR's Best Books of the Year "Expert storytelling . . . [Pollan] masterfully elevates a series of big questions about drugs, plants and humans that are likely to leave readers thinking in new ways." -New York Times Book Review From #1 New York Times bestselling author Michael Pollan, a radical challenge to how we think about drugs, and an exploration into the powerful human attraction to psychoactive plants-and the equally powerful taboos. Of all the things humans rely on plants for-sustenance, beauty, medicine, fragrance, flavor, fiber-surely the most curious is our use of them to change consciousness: to stimulate or calm, fiddle with or completely alter, the qualities of our mental experience. Take coffee and tea: People around the world rely on caffeine to sharpen their minds. But we do not usually think of caffeine as a drug, or our daily use as an addiction, because it is legal and socially acceptable. So, then, what is a "drug"? And why, for example, is making tea from the leaves of a tea plant acceptable, but making tea from a seed head of an opium poppy a federal crime? In This Is Your Mind on Plants, Michael Pollan dives deep into three plant drugs-opium, caffeine, and mescaline-and throws the fundamental strangeness, and arbitrariness, of our thinking about them into sharp relief. Exploring and participating in the cultures that have grown up around these drugs while consuming (or, in the case of caffeine, trying not to consume) them, Pollan reckons with the powerful human attraction to psychoactive plants. Why do we go to such great lengths to seek these shifts in consciousness, and then why do we fence that universal desire with laws and customs and fraught feelings? In this unique blend of history, science, and memoir, as well as participatory journalism, Pollan examines and experiences these plants from several very different angles and contexts, and shines a fresh light on a subject that is all too often treated reductively-as a drug, whether licit or illicit. But that is one of the least interesting things you can say about these plants, Pollan shows, for when we take them into our bodies and let them change our minds, we are engaging with nature in one of the most profound ways we can. Based in part on an essay published almost twenty-five years ago, this groundbreaking and singular consideration of psychoactive plants, and our attraction to them through time, holds up a mirror to our fundamental human needs and aspirations, the operations of our minds, and our entanglement with the natural world.

The Science of Grapevines: Anatomy and Physiology is an introduction to the physical structure of the grapevine, its various organs, their functions and their interactions with the environment. Beginning with a brief overview of the botanical classification (including an introduction to the concepts of species, cultivars, clones, and rootstocks), plant morphology and anatomy, and growth cycles of grapevines, The Science of Grapevines covers the basic concepts in growth and development, water relations, photosynthesis and respiration, mineral uptake and utilization, and carbon partitioning. These concepts are put to use to understand plant-environment interactions including canopy dynamics, yield formation, and fruit composition, and concludes with an introduction to stress physiology, including water stress (drought and flooding), nutrient deficiency and excess, extreme temperatures (heat and cold), and the impact and response to of other organisms. Based on the author's years of teaching grapevine anatomy as well as his research experience with grapevines and practical experience growing grapes, this book provides an important guide to understanding the entire plant.

"Principles of Soil and Plant Water Relations, 2e" describes the principles of water relations within soils, followed by the uptake of water and its subsequent movement throughout and from the plant body. This is presented as a progressive series of physical and biological interrelations, even though each topic is treated in detail on its own. The book also describes equipment used to measure water in the soil-plant-atmosphere system. At the end of each chapter is a biography of a scientist whose principles are discussed in the chapter. In addition to new information on the concept of celestial time, this new edition also includes new chapters on methods to determine sap flow in plants dual-probe heat-pulse technique to monitor water in the root zone.
Provides the necessary understanding to address advancing problems in water availability for meeting ecological requirements at local, regional and global scalesCovers plant anatomy: an essential component to understanding soil and plant water relations"