Published in 1975: This volume contains the completed section of the Handbook of Biochemistry and Molecular Biology with data pertaining to Lipids, Carbohydrates, and Steroids.

This book offers a new physical chemistry perspective on the control of lipid oxidation reactions by antioxidants, and it further explores the application of several oxidation inhibition strategies on food and biological systems. Divided in 3 parts, the book reviews the latest methods to control lipid oxidation, it examines lipid oxidation and inhibition in different food systems, and it finishes with an overview of the biological, health and nutritional effects of lipid oxidation. Chapters from expert contributors cover topics such as the use of magnetic methods to monitor lipid and protein oxidation, the kinetics and mechanisms of lipid oxidation and antioxidant inhibition reactions, interfacial chemistry, oxidative stress and its impact in human health, nutritional, sensory and physiological aspects of lipid oxidation, and new applications of plant and marine antioxidants. While focused on lipid peroxidation in food and biological systems, the chemistry elucidated in this book is applicable also to toxicology, medicine, plant physiology and pathology, and cosmetic industry. The book will therefore appeal to researchers in the lipid oxidation field covering food, biological and medical areas.

Lipobiology is a rapidly evolving interdisciplinary field which incorporates critical aspects of lipid and lipoprotein chemistry into the disciplines of cell biology and physiology. This series focuses on salient aspects of the role of lipids in metabolic regulation and cellular activation.

Angiogenesis has recently played a critical role in regulation of adipose tissue expansion and regression. Like most other tissues in the body, adipose expansion and regression is accompanied by alteration of blood vessel density and structures. The vascular alteration plays an active role in regulation of adipose tissue size and functions. Targeting blood vessels in the adipose tissue have demonstrated to be a novel approach for possibly treatment of cancer, obesity and other metabolic diseases. This book provides the most updated information on this type research and discusses future opportunities for therapy..

This detailed volume presents a variety of specialized analytical methods on a wide range of glycolipids. From sphingoglycolipids to lipid A, an immune system stimulant present in lipopolysaccharide, the book delves into how glycolipid structure on the surface of the cell membrane, which characterizes each organism, is crucial for the coexistence or repulsion between organisms. In addition, glycolipids play an important role in infections in animals and other organisms by targeting host cell membranes, if enveloped viruses are considered as living organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Glycolipids: Methods and Protocols serves as an ideal guide to the most advanced glycolipid research in an effort to help researchers make their research more fruitful.

Featuring practical strategies and exciting experiments, Teaching Innovations in Lipid Science addresses lipid education at a range of levels from the novice to the graduate student and teacher. Peer-reviewed contributions from internationally known specialists, describe several methods and approaches designed to create new lipid courses, modify existing courses, and serve as a basis for pursuing novel avenues of instruction. Divided into two sections, the first focuses on teaching strategies and outlines some of the barriers that lipid science specialists face when transmitting accurate information. It emphasizes the development and implementation of creative programs that foster interest in lipid science, and presents novel problem-solving approaches. It discusses strategies for involving and evaluating independent study students and explains the successful use of sample cards to teach oilseed and cereal processing. This section also provides generalized accounts of biotechnology and crop improvement and isoprenoid biochemistry, including improvement of oilseed crops and tips on explaining DNA science and crop biotechnology. The second section begins with simple demonstrations on the physical properties of lipids suitable for middle- and high school students. It follows with more complex experiments on analyzing lipids in food oils, plasma, and milk utilizing thin layer chromatography, gas chromatography, and high performance liquid chromatography. Contributions include information on convenient enzyme test kits with exercises that can translate to a lab course beginning with chromatographic methods for lipid analysis. The final chapter presents theory and experiments for studying lipid metabolism in the plastid by describing preparation methods, studying metabolite uptake, and pathway analysis.

Each of these chapters has a general section that describes the special needs for glycerolipid synthesis and the physiological context in which the regulation of phosphatidate phosphohydrolase activity can be understood.

Each of these chapters has a general section that describes the special needs for glycerolipid synthesis and the physiological context in which the regulation of phosphatidate phosphohydrolase activity can be understood.

This book is written by international experts with both academic and industrial credentials. It presents for the first time, a collection of up- to- date scientific advances in the area of edible fats and oils technology, over a span of 10 years from 2009 to the year 2019. The book covers the existing and recent advanced techniques adopted in the edible fats and oils research and touches on the processing and modification, to the traceability and sustainability issues of fats and oils. Some recent technologies like supercritical carbon dioxide, microwave, ultrasound, and enzymatic- assisted processes, ionic liquid, enzymatic processes, biosensor and membrane technology are presented in the book. The book aims to provide the technologists and researchers in research, development and operations in the edible fats and oils industries with critical and readily accessible information on the recent advances in the field. The book is divided into three broad sections- Fats and Oils Chemistry and Processing, Fats and Oils Modification and Health Implications, and Fats and Oils Safety, Social, Environmental and Economic Impacts. It is an extremely comprehensive and valuable resource, which serves as an essential reference for students and lipid scientists from academia or industry.

Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of proteina "lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.

The scientific advances in the physiology and pathophysiology of adipose tissue over the last two decades have been considerable. Today, the cellular and molecular mechanisms of adipogenesis are well known. In addition, adipose tissue is now recognized as a real endocrine organ that produces hormones such as the leptin acting to regulate food intake and energy balance in the central nervous system, a finding that has completely revolutionized the paradigm of energy homeostasis. Other adipokines have now been described and these molecules are taking on increasing importance in physiology and pathophysiology. Moreover, numerous works have shown that in obesity, but also in cases of lipodystophy, adipose tissue was the site of a local low-grade inflammation that involves immune cells such as macrophages and certain populations of lymphocytes. This new information is an important step in the pathophysiology of both obesity and related metabolic and cardiovascular complications. Finally, it is a unique and original work focusing on adipose tissue, covering biology and pathology by investigating aspects of molecular and cellular biology, general, metabolic, genetic and genomic biochemistry.

The 12th International Symposium on Plant Lipids was held at the University of Toronto, Canada, from July 7th to 12th, 1996. The conference was attended by over 200 scientists from university, government and corporate laboratories from 24 different countries. The topics covered in the symposium ranged from basic physiology, biochemistry and molecular biology of plant lipids to transformation and genetic engineering of crop plants. Oil seed plants were a particular focus of the symposium. There were 62 oral and 96 posters presentations. A special lecture in memory of the founder of this series of symposium, Terry Galliard, was presented by John Shanklin. This Proceedings Book has been dedicated to Grattan Roughan for his important contributions to our knowledge of plant lipid metabolism. This volume contains manuscripts submitted from most of the presentations at the symposium. It provides a useful summary of the major fields of plant lipid studies and our present state of knowledge. The papers are arranged in eight sections covering the major areas in the field of plant physiology, biochemistry and molecular biology of plant lipids. We would like to thank Valerie Imperial, Rajesh Khetarpal and Mary Williams for their invaluable help in organizing and running the meetings and excursions. John P. Williams, Mobashsher U. Khan and Nora W. Lem Toronto, Canada, October 1996 xvii DEDICATION This volume is dedicated to Grattan Roughan.

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine.

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

For the 6th Edition of this highly regarded textbook devoted to lipids, the title has been modified from Lipid Biochemistry to Lipids to acknowledge the coming together of biological and medical sciences, the increasingly blurred boundaries between them and the growing importance of lipids in diverse aspects of science and technology. The principal aims of this new edition - to inform students and researchers about lipids, to assist teachers and encourage further research have not changed since previous editions. Significant advances in lipid science have demanded yet another extensive rewriting for this edition, with the addition of two new authors, to cover new knowledge of genes coding for proteins involved in lipid metabolism, the many lipids involved in cell signalling, the roles of lipids in health and disease and new developments in biotechnology in support of agriculture and industry. An introductory chapter summarizes the types of lipids covered and their identification and provides a guide to the contents. Chapters contain boxes illustrating special topics, key point summaries and suggested further reading. Lipids: Sixth Edition provides a huge wealth of information for upper-level students of biological and clinical sciences, food science and nutrition, and for professionals working in academic and industrial research. Libraries in all universities and research establishments where biological, medical and food and nutritional sciences are studied and taught should have copies of this excellent and comprehensive new edition on their shelves.

Published in 1975: This volume contains the completed section of the Handbook of Biochemistry and Molecular Biology with data pertaining to Lipids, Carbohydrates, and Steroids.

New research tools have revealed many surprising aspects of the dynamic nature of lipids and their participation in processes such as recognition, intra- and inter-cellular signalling, deterrence and defence against pathogens, membrane trafficking and protein function. This is in addition to new information on the more established roles of plant lipids as structural components of membranes and as long-term storage products. Plant lipids are also increasingly being seen as sources of a new generation of environmentally friendly, biodegradable and renewable industrial products, including biopolymers and high grade lubricants.

This volume provides a broad overview of plant lipid research and its many applications, linking the various disciplines and providing an interesting and wide-ranging perspective on this fast-moving field. Extensive lists of references are provided, totalling well over two thousand non-redundant citations and offering a point of entry to the detailed literature.

This is a book for researchers and professionals in plant biochemistry, molecular biology, biotechnology and genetics, in both the academic and industrial sectors.

The present book gives a multi-disciplinary perspective on the physics of life and the particular role played by lipids (fats) and the lipid-bilayer component of cell membranes. The emphasis is on the physical properties of lipid membranes seen as soft and molecularly structured interfaces. By combining and synthesizing insights obtained from a variety of recent studies, an attempt is made to clarify what membrane structure is and how it can be quantitatively described. Furthermore, it is shown how biological function mediated by membranes is controlled by lipid membrane structure and organization on length scales ranging from the size of the individual molecule, across molecular assemblies of proteins and lipid domains in the range of nanometers, to the size of whole cells. Applications of lipids in nanotechnology and biomedicine are also described. The first edition of the present book was published in 2005 when lipidomics was still very much an emerging science and lipids about to be recognized as being as important for life as proteins, sugars, and genes. This significantly expanded and revised edition takes into account the tremendous amount of knowledge gained over the past decade. In addition, the book now includes more tutorial material on the biochemistry of lipids and the principles of lipid self-assembly. The book is aimed at undergraduate students and young research workers within physics, chemistry, biochemistry, molecular biology, nutrition, as well as pharmaceutical and biomedical sciences. From the reviews of the first edition: "This is a highly interesting book and a pleasure to read. It represents a new and excellent pedagogical introduction to the field of lipids and the biophysics of biological membranes. I reckon that physicists and chemists as well as biologists will benefit from this approach to the field and Mouritsen shows a deep insight into the physical chemistry of lipids." (Goeran Lindblom, Chemistry and Physics of Lipids 2005, vol. 135, page 105-106) "The book takes the reader on an exciting journey through the lipid world, and Mouritsen attracts the attention with a lively style of writing ... . a comprehensive view of the 'lipid sea' can be easily achieved, gaining the right perspectives for envisaging future developments in the nascent field of lipidomics." (Carla Ferreri, ChemBioChem, Vol. 6 (8), 2005)

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology (TM) series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field.

The lipids of cellular membranes not only serve roles in controlling the structure and fluidity of the membrane, but are increasingly recognized for their roles as signalling molecules and modifiers of membrane protein function. Recent studies described in this volume reveal striking changes in membrane lipids during aging and in age-related diseases such as cancer, cardiovascular disease and neurodegenerative disorders. Lipids including inositol phospholipids, cholesterol, sphingolipids and ceramides play important roles in signalling cellular responses to stress and specific stimuli such as growth factors, cytokines and neurotransmitters. One or more of these lipid mediators has been linked to the pathogenesis of age-related diseases.

This book provides a comprehensive review of specific membrane lipid mediators and their roles in aging and age-related disease.

This is the second volume in a series which presents the state of the art in chosen sectors of oil and fat chemistry, including its relevance to the food and pharmaceutical industries. The text in this book aims to provide an authoritative account of the use of a wide range of spectroscopic methods in the analysis of lipids, with an emphasis on topics that have attracted special attention.

The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation. This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures. A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.

Leading international scientists bring current and developing topics in sterol research together in Biochemistry and Function of Sterols. The authors are experts in each major area of sterol research-medicine, biochemistry, chemistry, and agriculture. Each chapter features the current state of research as well as new and developing research topics. Throughout the volume the focus is on the major and expanding areas of sterol biochemistry and function of sterols in all classes or organisms. The broad scope of this work embraces many disciplines and will be of interest to a variety of researchers, students, and lay people. Professors will find Biochemistry and Function of Sterols an excellent choice as a textbook for courses on steroid, lipid, or plant biochemistry.

There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques curre