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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Lipids
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Tandem Mass Spectrometry of Lipids - Molecular Analysis of Complex Lipids (Hardcover)
Loot Price: R4,908
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Tandem Mass Spectrometry of Lipids - Molecular Analysis of Complex Lipids (Hardcover)
Series: New Developments in Mass Spectrometry, Volume 4
Expected to ship within 10 - 15 working days
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The emerging field of lipidomics has been made possible because of
advances in mass spectrometry, and in particular tandem mass
spectrometry of lipid ions generated by electrospray ionization.
The ability to carry out basic biochemical studies of lipids using
electrospray ionization is predicated upon understanding the
behaviour of lipid derived ions following collision induced
decomposition and mechanisms of product ion formation. During the
past 20 years, a wealth of information has been generated about
lipid molecules that are now analysed by mass spectrometry, however
there is no central source where one can obtain basic information
about how these very diverse biomolecules behave following
collisional activation. This book brings together, in one volume,
this information so that investigators considering using tandem
mass spectrometry to structurally characterize lipids or to
quantitate their occurrence in a biological matrix, will have a
convenient source to review mechanism of decomposition reactions
related to the diversity of lipid structures. A separate chapter is
devoted to each of seven major lipid classes including fatty acids,
eicosanoids and bioactive lipid mediators, fatty acyl esters and
amides, glycerol esters, glycerophospholipids, sphingolipids, and
steroids. Mechanistic details are provided for understanding the
pathways of formation of major product ions and ions used for
structural characterization. In most cases specific ancillary
information has been critical to understand the pathways, including
isotope labeling and high resolution analysis of precursor and
product ions. For a few specific examples such data is missing and
pathways are proposed as a means to initiate further mass spectral
experiments to prove or disprove pathway hypotheses. While this
work largely centres on the lipid biochemistry of animal
(mammalian) systems, general principles can be taken from the
specific examples and applied to lipid biochemistry found in
plants, fungi, prokaryotes and archeal organisms.
General
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