This book describes the basic botanical features of kiwifruit and its wild relatives, reports on the steps that led to its genome sequencing, and discusses the results obtained with the assembly and annotation. The core chapters provide essential insights into the main gene families that characterize this species as a crop, including the genes controlling sugar and starch metabolism, pigment biosynthesis and degradation, the ascorbic-acid pathway, fruit softening and postharvest metabolism, allergens, and resistance to pests and diseases. The book offers a valuable reference guide for taxonomists, geneticists and horticulturists. Further, since information gained from the genome sequence is extraordinarily useful in assessing the breeding value of individuals based on whole-genome scans, it will especially benefit plant breeders. Accordingly, chapters are included that focus on gene introgression from wild relatives and genome-based breeding.

This book is a compilation of recent global measures to conserve bio-resources and manage biotic and abiotic stresses. It highlights emerging issues related to agriculture, abiotic and biotic stress factors, ethnic knowledge, climate change and global warming, as well as natural resources and their sustainable management. It also focuses on the consolidated efforts of scientists and academics engaged in addressing a number of issues related to resource management and combating stresses in order to protect the Earth. Crop production and productivity have been significantly improved, however, there have been no corresponding practical advances in sustainable agriculture.This book offers a wide range of affordable approaches to managing bio-resources with a focus on sustainability. Lastly, it describes research highlights and future areas of research.

The Argentinean Patagonia offers a great diversity of scarcely explored environments suitable for the bioprospection of biotechnological relevant microorganisms. This book provides readers with a concise and clearly illustrated treatment of outstanding topics of Patagonian microbiology and biotechnology. It covers a wide range of areas interesting to several audiences such as researchers, graduate students and professionals working on the industry food. Among the main topics we will discuss examples of environmental applications, such as heavy metal and hydrocarbon bioremediation, bioprospection of valuable molecules from extremophilic bacteria and yeasts, the use of Patagonian yeasts and lactic acid bacteria in fermented foods and beverages, aquaculture probiotics and yeasts for food biopreservation.

This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

This book highlights the implications of nanotechnology in plant sciences, particularly its potential to improve food and agricultural systems, through innovative, eco-friendly approaches, and as a result to increase plant productivity. Topics include various aspects of nanomaterials: biophysical and biochemical properties; methods of treatment, detection and quantification; methods of quantifying the uptake of nanomaterials and their translocation and accumulation in plants. In addition, the effects on plant growth and development, the role of nanoparticles in changes in gene and protein expression, and delivery of genetic materials for genetic improvement are discussed. It also explores how nanotechnology can improve plant protection and plant nutrition, and addresses concerns about using nanoparticles and their compliances. This book provides a comprehensive overview of the application potential of nanoparticles in plant science and serves as a valuable resource for students, teachers, researchers and professionals working on nanotechnology.

Fungi are distinct eukaryotic organisms renowned for their remarkable biodiversity and extensive habitat range. Many fungal species have long been exploited for food and medicines. This volume considers other important applications of fungal biotechnology especially in an environmental context, showcasing the essential contributions of these amazingly versatile organisms. It explores how fungi offer sustainable solutions to tackle various environmental concerns. Written by eminent experts in their fields, this work presents a broad array of current advances and future prospects in fungal environmental biotechnology and discusses their limitations and potential. The book is organized in five parts, each addressing a theme of the UN Sustainable Development Goals (SDG): strengthen food security (Zero Hunger), wastewater treatment (Clean Water & Sanitation), pollution reduction (Life on Land), biofuel production (Affordable & Clean Energy) and biosynthesis of novel biomolecules (Responsible Consumption & Production).

This book presents a flavour of activities focussed on the need for sustainably produced biomass to support European strategic objectives for the developing bioeconomy. The chapters cover five broad topic areas relating to the use of perennial biomass crops in Europe. These are: 'Bioenergy Resources from Perennial Crops in Europe', 'European Regional Examples for the Use of Perennial Crops for Bioenergy', 'Genotypic Selection of Perennial Biomass Crops for Crop Improvement', 'Ecophysiology of Perennial Biomass Crops' and 'Examples of End-Use of Perennial Biomass Crops'. Two major issues relating to the future use of biomass energy are the identification of the most suitable second generation biomass crops and the need to utilise land not under intensive agricultural production, broadly referred to as 'marginal land'. The two main categories of plants that fit these needs are perennial rhizomatous grasses and trees that can be coppiced. The overarching questions that are addressed in the book relate to the suitability of perennial crops for providing feedstocks for a European bioeconomy and the need to exploit environments for biomass crops which do not compete with food crops. Bioenergy is the subject of a wide range of national and European policy measures. New developments covered are, for example, the use of perennial grasses to produce protein for animal feed and concepts to use perennial biomass crops to mitigate carbon emissions through soil carbon sequestration. Several chapters also show how prudent selection of suitable genotypes and breeding are essential to develop high yielding and sustainable second generation biomass crops which are adapted to a wide range of unfavourable conditions like chilling and freezing, drought, flooding and salinity. The final chapters also emphasise the need to be kept an eye out for potential new end-uses of perennial biomass crops that will contribute further to the developing bioeconomy.

Drought is one of the most severe constraints to crop productivity worldwide, and thus it has become a major concern for global food security. Due to an increasing world population, droughts could lead to serious food shortages by 2050. The situation may worsen due to predicated climatic changes that may increase the frequency, duration and severity of droughts. Hence, there is an urgent need to improve our understanding of the complex mechanisms associated with drought tolerance and to develop modern crop varieties that are more resilient to drought. Identification of the genes responsible for drought tolerance in plants will contribute to our understanding of the molecular mechanisms that could enable crop plants to respond to drought. The discovery of novel drought related genes, the analysis of their expression patterns in response to drought, and determination of the functions these genes play in drought adaptation will provide a base to develop effective strategies to enhance the drought tolerance of crop plants. Plant breeding efforts to increase crop yields in dry environments have been slow to date mainly due to our poor understanding of the molecular and genetic mechanisms involved in how plants respond to drought. In addition, when it comes to combining favourable alleles, there are practical obstacles to developing superior high yielding genotypes fit for drought prone environments. Drought Tolerance in Plants, Vol 2: Molecular and Genetic Perspectives combines novel topical findings, regarding the major molecular and genetic events associated with drought tolerance, with contemporary crop improvement approaches. This volume is unique as it makes available for its readers not only extensive reports of existing facts and data, but also practical knowledge and overviews of state-of-the-art technologies, across the biological fields, from plant breeding using classical and molecular genetic information, to the modern omic technologies, that are now being used in drought tolerance research to breed drought-related traits into modern crop varieties. This book is useful for teachers and researchers in the fields of plant breeding, molecular biology and biotechnology.

This book examines the application of soybean genome sequences to comparative, structural, and functional genomics. Since the availability of the soybean genome sequence has revolutionized molecular research on this important crop species, the book also describes how the genome sequence has shaped research on transposon biology and applications for gene identification, tilling and positional gene cloning. Further, the book shows how the genome sequence influences research in the areas of genetic mapping, marker development, and genome-wide association mapping for identifying important trait genes and soybean breeding. In closing, the economic and botanical aspects of the soybean are also addressed.

Setaria viridis and S.italica make up a model grass system to investigate C4 photosynthesis, cell wall biosynthesis, responses to drought, herbicide, and other environmental stressors, genome dynamics, developmental genetics and morphology, and interactions with microorganisms. Setaria viridis (green foxtail) is one of the world's most widespread weeds, and its small size, native variation, rapidly burgeoning genetic and genomic resources, and transformability are making it the system of choice for both basic research and its translation into crop improvement. Its domesticated variant, S. italica (foxtail millet), is a drought-hardy cereal grown in China, India and Africa, and new breeding techniques show great potential for improving yields and nutrition for drought-prone regions. This book brings together for the first time evolutionary, genomic, genetic, and morphological analyses, together with protocols for growing and transforming Setaria, and approaches to high throughput genotyping and candidate gene analysis. Authors include major Setaria researchers from both the USA and overseas.

This book provides insights into the latest achievements in genomics research on Brassica rapa. It describes the findings on this Brassica species, the first of the U's triangle that has been sequenced and a close relative to the model plant Arabidopsis, which provide a basis for investigations of major Brassica crop species. Further, the book focuses on the development of tools to facilitate the transfer of our rich knowledge on Arabidopsis to a cultivated Brassica crop. Key topics covered include genomic resources, assembly tools, annotation of the genome, transposable elements, comparative genomics, evolution of Brassica genomes, and advances in the application of genomics in the breeding of Brassica rapa crops.

This book is devoted to grain legumes and include eight chapters devoted to the breeding of specific grain legume crops and five general chapters dealing with important topics which are common to most of the species in focus. Soybean is not included in the book as it is commonly considered an oil crop more than a grain legume and is included in the Oil Crops Volume of the Handbook of Plant Breeding. Legume species belong to the Fabaceae family and are characterized by their fruit, usually called pod. Several species of this family were domesticated by humans, such as soybean, common bean, faba bean, pea, chickpea, lentil, peanut, or cowpea. Some of these species are of great relevance as human and animal food. Food legumes are consumed either by their immature pod or their dry seeds, which have a high protein content. Globally, grain legumes are the most relevant source of plant protein, especially in many countries of Africa and Latin America, but there are some constraints in their production, such as a poor adaptation, pest and diseases and unstable yield. Current research trends in Legumes are focused on new methodologies involving genetic and omic studies, as well as new approaches to the genetic improvement of these species, including the relationships with their symbiotic rhizobia.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

This important 2-volume reference book is the first comprehensive resource reflecting the current global status and prospects of date palm cultivation by country. This volume covers Africa and the Americas. Countries included are: Egypt, Algeria, Sudan, Tunisia, Libya, Morocco, Mauritania, Niger, Cameroon, Djibouti, Chad, Mali Somalia, Ethiopia, Burkina Faso and Senegal, as well as the United States of America and the South American countries Chile and Peru. Topics discussed are: cultivation practices; genetic resources and breeding; conservation and germplasm banks; cultivar classification and identification based on morphological and molecular markers; micropropagation and progress toward scale-up production; and advances in dates processing and marketing. Chapters are supported by tables and color photographs. Appendixes summarize traits and distribution of major cultivars, commercial resources of offshoots and in vitro plants; and institutions and scientific societies concerned with date palm.

This proceeding covers all the collected research data and presentations from the 8th International Symposium on the Molecular Breeding of Forage and Turf. The book explores themes in molecular breeding of forage and turf, including abiotic and biotic stresses, bioenergy and biorenewables, comparative genomics, emerging tools for forage and turf research, functional genetics and genomics and genetic mapping germplasm, diversity and its impact on breeding, herbage quality, plant-microbe interactions and transgenic and risk assessment. Written by renowned researchers in plant genomics, Molecular Breeding of Forage and Turf: The Proceedings of the 8th International Symposium on the Molecular Breeding of Forage and Turf is a valuable resource for researchers and students in the field of plant genomics.

This book describes the various applications of microorganisms in improving plant growth, health and the efficiency of phytochemical production. The chapters trace topics such as the role of PGPRs in improving salt stress and heavy metal tolerance in plants; the prevention and control of plant diseases; boosting soil fertility and agriculture productivity; the induction of secondary metabolite biosynthesis in medicinal and aromatic plants; the enhancement of phytochemical levels, and the action mechanisms, diversity and characterization of PGPRs. The reviews will be of interest for scientists in the fields of agriculture, microbiology, soil biology, plant breeding and herbal medicinal products.

This book presents a holistic view of the complex and dynamic responses of plants to nanoparticles, the signal transduction mechanisms involved, and the regulation of gene expression. Further, it addresses the phytosynthesis of nanoparticles, the role of nanoparticles in the antioxidant systems of plants and agriculture, the beneficial and harmful effects of nanoparticles on plants, and the application of nanoparticles and nanotubes to mass spectrometry, aiming ultimately at an analysis of the metabolomics of plants. The growing numbers of inventions in the field of nanotechnology are producing novel applications in the fields of biotechnology and agriculture. Nanoparticles have received much attention because of the unique physico-chemical properties of these compounds. In the life sciences, nanoparticles are used as “smart” delivery systems, prompting the Nobel Prize winner P. Ehrlich to refer to these compounds as “magic bullets.” Nanoparticles also play an important role in agriculture as compound fertilizers and nano-pesticides, acting as chemical delivery agents that target molecules to specific cellular organelles in plants. The influence of nanoparticles on plant growth and development, however, remains to be investigated. Lastly, this book reveals the research gaps that must be bridged in the years to come in order to achieve larger goals concerning the applications of nanotechnology in the plants sciences. In the 21st century, nanotechnology has become a rapidly emerging branch of science. In the world of physical sciences, nanotechnological tools have been exploited for a broad range of applications. In recent years, nanoparticles have also proven useful in several branches of the life sciences. In particular, nanotechnology has been employed in drug delivery and related applications in medicine.

This book represents a pioneer initiative to describe the new technologies available for next-generation phenotyping and applied to plant breeding. Over the last several years plant breeding has experienced a true revolution. Phenomics, i.e., high-throughput phenotyping using automation, robotics and remote data collection, is changing the way cultivars are developed. Written in an easy to understand style, this book offers an indispensable reference work for all students, instructors and scientists who are interested in the latest innovative technologies applied to plant breeding.

White rust caused by the fungus Albugo is the most devastating disease known to occur in more than 50 countries and infects about 400 plant species belonging to 31 families worldwide including important vegetable crucifers, oil yielding Brassicas, ornamental plants and numerous weeds. This book on "White Rust" deals with the aspects on "the disease" and "the pathogen" is vividly illustrated for stimulating, effective and easy reading and understanding. We are sure that this comprehensive treatise on "white rust" will be of immense use to the researchers, teachers, students and all others who are interested in the diagnosis and management of white rust diseases of crops worldwide.

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one's own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O'Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.

This book describes how genomics has revolutionized our understanding of agriculturally important plant-associated fungi and oomycetes. It illustrates some fundamental discoveries about these eukaryotic microbes with regard to the overall structure of their genomes, their lifestyles and the molecular mechanisms that form the basis of their interactions with plants. Genomics has provided new insights into fungal lifestyles and led to practical advances in plant breeding and crop protection, such as predictions about the spread and evolution of new pathogens.This volume focuses on fungi and oomycetes that are typical dicot plant pathogens and includes: Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria sp.,Verticillium alfalfae and Verticillium dahliae, Fusarium oxysporum, Phytophthora capsici, Phytophthora sojae, Phytophthora ramorum, Phytophthora infestans, Hyaloperonospora arabidopsidis.

This volume presents the issues and challenges of crop pathogens and plant protection. Composed of the latest knowledge in plant pathology, the book covers topics such as fungal diseases of the groundnut, plant growth promoting rhizobacteria, plant pathogenic fungi in the genomics era, the increased virulence of wheat rusts and oat fungal diseases. Written by experienced and internationally recognized scientists in the field, Future Challenges in Crop Protection Against Fungal Pathogens is a concise yet comprehensive resource valuable for both novice as well as experienced plant scientists and researchers.

The field of plant breeding has grown rapidly in the last decade with breakthrough research in genetics and genomics, inbred development, population improvement, hybrids, clones, self-pollinated crops, polyploidy, transgenic breeding and more. This book discusses the latest developments in all these areas but explores the next generation of needs and discoveries including omics beyond genomics, cultivar seeds and intellectual and property rights. This book is a leading-edge publication of the latest results and forecasts important areas of future needs and applications.

This book provides detailed and comprehensive information on oxidative damage caused by stresses in plants with especial reference to the metabolism of reactive oxygen species (ROS). In plants, as in all aerobic organisms, ROS are common by-products formed by the inevitable leakage of electrons onto O2 from the electron transport activities located in chloroplasts, mitochondria, peroxisomes and in plasma membranes or as a consequence of various metabolic pathways confined in different cellular loci. Environmental stresses such as heat, cold, drought, salinity, heavy-metal toxicity, ozone and ultraviolet radiation as well as pathogens/contagion attack lead to enhanced generation of ROS in plants due to disruption of cellular homeostasis. ROS play a dual role in plants; at low concentrations they act as signaling molecules that facilitate several responses in plant cells, including those promoted by biotic and abiotic agents. In divergence, at high levels they cause damage to cellular constituents triggering oxidative stress. In either case, small antioxidant molecules and enzymes modulate the action of these ambivalent species.

This book provides insights into some of the key achievements made in the study of Lotus japonicus (birdsfoot trefoil), as well as a timely overview of topics that are pertinent for future developments in legume genomics. Key topics covered include endosymbiosis, development, hormone regulation, carbon/nitrogen and secondary metabolism, as well as advances made in high-throughput genomic and genetic approaches. Research focusing on model plants has underpinned the recent growth in plant genomics and genetics and provided a basis for investigations of major crop species. In the legume family Fabaceae, groundbreaking genetic and genomic research has established a significant body of knowledge on Lotus japonicus, which was adopted as a model species more than 20 years ago. The diverse nature of legumes means that such research has a wide potential and agricultural impact, for example, on the world’s protein production.