Nanotechnology is the application of science to control matter at the molecular level. It has become one of the most promising applied technologies in all areas of science. Nanoparticles have multi-functional properties and have created very interesting applications in various fields such as medicine, nutrition, bioenergy, agriculture and the environment. But the biogenic syntheses of monodispersed nanoparticles with specific sizes and shapes have been a challenge in biomaterial science. Nanoparticles are of great interest due to their extremely small size and large surface-to-volume ratio, which lead to both chemical and physical differences in their properties (e.g., mechanical properties, biological and sterical properties, catalytic activity, thermal and electrical conductivity, optical absorption and melting point) compared to bulk of the same chemical composition. Recently, however, synthesizing metal nanoparticles using green technology via microorganisms, plants, viruses, and so on, has been extensively studied and has become recognized as a green and efficient way for further exploiting biological systems as convenient nanofactories. Thus the biological synthesis of nanoparticles is increasingly regarded as a rapid, ecofriendly, and easily scaled-up technology. Today researchers are developing new techniques and materials using nanotechnology that may be suitable for plants to boost their native functions. Recently, biological nanoparticles were found to be more pharmacologically active than physico-chemically synthesized nanoparticles. Various applications of biosynthesized nanoparticles have been discovered, especially in the field of biomedical research, such as applications to specific delivery of drugs, use for tumor detection, angiogenesis, genetic disease and genetic disorder diagnosis, photoimaging, and photothermal therapy. Further, iron oxide nanoparticles have been applied to cancer therapy, hyperthermia, drug delivery, tissue repair, cell labeling, targeting and immunoassays, detoxification of biological fluids, magnetic resonance imaging, and magnetically responsive drug delivery therapy. Nanoparticle synthesis for plant byproducts for biomedical applications has vast potential. This book offers researchers in plant science and biomedicine the latest research and opportunity to develop new tools for the synthesis of environmentally friendly and cost-effective nanoparticles for applications in biomedicine as well as other various fields.

Our knowledge of pollen, the gold dust that carries the male germ line of flowering plants and is vital for sexual reproduction and seed formation, has 'come of age' with the publication of this book. Here, for the first time in a single volume, are all the ideas and techniques developed in the last two decades concerning the manipulation of pollen and pollen tubes in plant breeding and biotechnology. Pollen has never been an easy topic to come to grips with, with its variable and often inexplicable terminology that has made it a more difficult field in which to work. This book will remedy that, with its overview of pollen biology and pollen-pistil interactions that explains terms and concepts of the male function of pollen in a way that is readily understandable to the student and professional plant scientist and plant breeder.

This book describes the current state of international grape genomics, with a focus on the latest findings, tools and strategies employed in genome sequencing and analysis, and genetic mapping of important agronomic traits. It also discusses how these are having a direct impact on outcomes for grape breeders and the international grape research community. While V. vinifera is a model species, it is not always appreciated that its cultivation usually requires the use of other Vitis species as rootstocks. The book discusses genetic diversity within the Vitis genus, the available genetic resources for breeding, and the available genomic resources for other Vitis species. Grapes (Vitis vinifera spp. vinifera) have been a source of food and wine since their domestication from their wild progenitor (Vitis vinifera ssp. sylvestris) around 8,000 years ago, and they are now the world's most valuable horticultural crop. In addition to being economically important, V. vinifera is also a model organism for the study of perennial fruit crops for two reasons: Firstly, its ability to be transformed and micropropagated via somatic embryogenesis, and secondly its relatively small genome size of 500 Mb. The economic importance of grapes made V. vinifera an obvious early candidate for genomic sequencing, and accordingly, two draft genomes were reported in 2007. Remarkably, these were the first genomes of any fruiting crop to be sequenced and only the fourth for flowering plants. Although riddled with gaps and potentially omitting large regions of repetitive sequences, the two genomes have provided valuable insights into grape genomes. Cited in over 2,000 articles, the genome has served as a reference in more than 3,000 genome-wide transcriptional analyses. Further, recent advances in DNA sequencing and bioinformatics are enabling the assembly of reference-grade genome references for more grape genotypes revealing the exceptional extent of structural variation in the species.

Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it will mostly focus on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. The third volume is titled In Silico Life Sciences: Agriculture. It focuses on plant genetic, genomic, transcriptomic, proteomic and metabolomics data. Using examples of new crop diseases-emergence, crop productivity and biotic/abiotic stress tolerance, this book illustrates how bioinformatics can be an integral components of modern day plant science research.

This book describes the sequencing efforts for Neem (Azadirachta indica A. Juss), one of the most versatile tropical evergreen tree species. The neem tree is a source of various natural products, including the potent biopesticide azadirachtin and limonoids, which have a broad spectrum of activity against insect pests and microbial pathogens. To identify genes and pathways in neem, three neem genomes and several transcriptomes are studied using next-generation sequencing technologies. Neem has been extensively used in Ayurveda, Unani and homoeopathic medicine and is often referred to as the 'village pharmacy' by natives due to its wealth of medicinal properties. Besides the description of the genome, this book discusses the neem microbiome and its role in the production of neem metabolites like salanin, nimbin and exopy-azadirachtin under in vitro conditions. It also highlights cell and tissue culture using various neem explants including the leaf, root, shoot, cambium, etc.

Attaining sustainable agricultural production while preserving environmental quality, agro-ecosystem functions and biodiversity represents a major challenge for current agricultural practices; further, the traditional use of chemical inputs (fertilizers, pesticides, nutrients etc.) poses serious threats to crop productivity, soil fertility and the nutritional value of farm produce. Given these risks, managing pests and diseases, maintaining agro-ecosystem health, and avoiding health issues for humans and animals have now become key priorities. The use of PGPR as biofertilizers, plant growth promoters, biopesticides, and soil and plant health managers has attracted considerable attention among researchers, agriculturists, farmers, policymakers and consumers alike. Using PGPR as bioinoculants can help meet the expected demand for global agricultural productivity to feed the world's booming population, which is predicted to reach roughly 9 billion by 2050. However, to provide effective bioinoculants, PGPR strains must be safe for the environment, offer considerable plant growth promotion and biocontrol potential, be compatible with useful soil rhizobacteria, and be able to withstand various biotic and abiotic stresses. Accordingly, the book also highlights the need for better strains of PGPR to complement increasing agro-productivity.

Over the last decade considerable progress has been made in white biotechnology research and further major scientific and technological breakthroughs are expected in the future. The first large-scale industrial applications of modern biotechnology have been in the areas of food and animal feed production (agricultural/green biotechnology) and in pharmaceuticals (medical/red biotechnology). In contrast, the productions of bioactive compounds through fermentation or enzymatic conversion are known as industrial or white biotchnology. The fungi are ubiquitous in nature and have been sorted out from different habitats, including extreme environments (high temperature, low temperature, salinity and pH); and associated with plants (Epiphytic, Endophytic and Rhizospheric). The fungal strains are beneficial as well as harmful for human beings. The beneficial fungal strains may play important roles in the agricultural, industrial, and medical sectors. The fungal strains and its product (enzymes, bioactive compounds, and secondary metabolites) are very useful for industry (e.g., the discovery of penicillin from Penicillium chrysogenum). This discovery was a milestone in the development of white biotechnology as the industrial production of penicillin and antibiotics using fungi moved industrial biotechnology into the modern era, transforming it into a global industrial technology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors providing both high value nutraceutical and pharmaceutical products. The fungal strains and bioactive compounds also play an important role in environmental cleaning. This volume covers the latest research developments related to value-added products in white biotechnology through fungi.

The scientific and technical development of any kind of germplasm is regulated by a vast network of treaties, conventions, international agreements, and national and regional legislation. These regulations govern biotechnological innovations in plants and microorganisms, access to and use of plant genetic resources, and biosafety. This complex mix has made it difficult to arrive at global interpretations, due to overlaps, gaps, ambiguities, contradictions, and lack of consistency. The big picture is even more complex, as a series of scientific developments - gene editing in particular - have in some cases rendered these international regulatory frameworks obsolete. This book puts forward an innovative approach: a "Comprehensive Plant Germplasm System". The System is a cooperative game theory-based proposal for a binding international convention which would supersede all other conventions, treaties, national and regional legislation covering native varieties and traditional developments, heterogeneous plant varieties, microorganisms, biotechnological inventions, plant genetic resources, and biosafety regulation. In short, it offers a comprehensive framework regarding intellectual property, biosafety, and business regulation and covers all types of germplasm. If applied, the system is expected to yield higher productivity rates in crops and improved food biodiversity, as well as a new paradigm based on the promotion of innovation for "Agriculture 4.0."

This book presents the life science experiments in a space microgravity environment conducted on board the SJ-10 recoverable satellite, which was launched on April 6th 2016 and recovered on April 18th 2016. It covers 10 scientific projects in radiation biology, gravitational biology and biotechnology that were selected from ~100 proposals from various institutions in China and around the world. Primarily exploring the rhythm of life in a space microgravity environment, all of the experiments - conducted on nine payloads of the SJ-10 satellite - have never been previously conducted in the respective fields. In addition, the book provides extensive information on the mission's execution, data collection, and scientific outcomes.

Natural remedies play an important role in curing ailments that happen by chance or adoptively. Adhatoda vasica is a natural Asian remedy that has the potential to treat respiratory disorders, among others. While existing literature does exist on this medicinal plant, it can be cumbersome to sort through for information relevant to academics, research, and practice. This work reframes and consolidates information on Adhatoda vasica so that it is easier to approach through academic, research, and practitioner perspectives.

This is the first book on the genome of Salvia miltiorrhiza, summarizing the research advances in the molecular mapping, whole genome sequencing, chloroplast and mitochondria genomes, epigenetics, transcriptomics and functional genomics of this emerging model plant with great economic and medicinal value. It also describes its distribution, taxonomy and morphology and provides useful information on its cultivation and breeding. Further, it highlights the biosynthetic pathways of tanshinones and phenolic acids - two main classes of bioactive components produced in this plant species - and reviews and discusses the technology of hairy root induction, tissue culture and genetic transformation of S. miltiorrhiza. The book is a valuable resource for students, teachers and researchers in academia and industry interested in medicinal plants and pharmacy.

Linum (flax) is a genus of about 200 species in the flowering plant family Linaceae. The genus includes common flax, which is one of the best fibers to produce linen, the seeds to produce linseed oil and has health-related properties of flax in human and animal nutrition. This book describes the genetics and genomics of Linum including the development of extensive experimental resources (e.g. whole genome sequence, efficient transformation methods, insertional mutant collections, large germplasm collections, resequenced genomes) that have led much progress and its economic importance. The methods and use of Linum to address a wide range of applications (e.g. disease resistance, cell wall composition, abiotic stress tolerance, floral development, natural diversity) is also discussed.

This book discusses the role of salt in current agricultural approaches, including the low salt tolerance of agricultural crops and trees, impact of saline soils, and salt-resistant plants. Halophytes are extremely salt tolerant plants, which are able to grow and survive under salt at concentrations as high as 5 g/l by maintaining negative water potential. The salt-tolerant microbes inhabiting the rhizospheres of halophytes may contribute to their salt tolerance, and the rhizospheres of halophytic plants provide an ideal opportunity for isolating various groups of salt-tolerant microbes that could enhance the growth of different crops under salinity stress. The book offers an overview of salt-tolerant microbes' ability to increase plant tolerance to salt to facilitate plant growth, the potential of the halophytes' rhizospheres as a reservoir of beneficial salt-tolerant microbes, their future application as bio-inoculants in agriculture and a valuable resource for an alternative way of improving crop tolerance to salinity and promoting saline soil-based agriculture. This special collection of reviews highlights some of the recent advances in applied aspects of plant (halophytes)-microbe interactions and their contribution towards eco-friendly approaches saline soil-based agriculture.

This edited volume summarizes the recent advancements made in plant science including molecular biology and genome editing , particularly in the development of novel pathways tolerant to climate change-induced stresses such as drought, extreme temperatures, cold, salinity, flooding, etc. These stresses are liable for decrease in yields in many crop plants at global level. Till date conventional plant breeding approaches have resulted in significant improvement of crop plants for producing higher yields during adverse climatic conditions. However, the pace of improvement through conventional plant breeding needs to be accelerated in keeping with the growing demand of food and increasing human populationl, particularly in developing world. This book serves as a comprehensive reference material for researchers, teachers, and students involved in climate change-related abiotic stress tolerance studies in plants.

Dothistroma pini changed New Zealand commercial forestry dramatically. Tree breeding became concentrated on a very few species and development of selection methods and breeding strategies changed in response to the new challenges. Tree-Breeding and Genetics in New Zealand provides a critical historical account of the work on provenance research and tree breeding, often with the wisdom of hindsight, and it tracks the development of breeding strategy, especially for P. radiata, Douglas-fir and the most important eucalypt species, E. regnans, E. fastigata and E. nitens. The book is a compendium of abstracts and summaries of all publications and reports on tree improvement in New Zealand since the early 1950s, with added critical comment by the author on much of the work. It is intended for other tree breeders internationally, for interested NZ foresters and for graduate students studying genetics and tree breeding.

Sustainable increase in agricultural production while keeping the environmental quality, agro-ecosystem function and biodiversity is a real challenge in current agricultural practices. Application of PGPR can help in meeting the expected demand for increasing agricultural productivity to feed the world's booming population. Global concern over the demerits of chemicals in agriculture has diverted the attention of researchers towards sustainable agriculture by utilizing the potential of Plant Growth Promoting Rhizobacteria (PGPR). Use of PGPR as biofertilizers, biopesticides, soil, and plant health managers has gained considerable agricultural and commercial significance. The book Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture has contributions in the form of book chapter from 25 eminent global researchers, that discusses about the PGPRs and their role in growth promotion of various crop plants, suppression of wide range of phytopathogens, their formulation, effect of various factors on growth and performance of PGPR, assessment of diversity of PGPR through microsatellites and role of PGPR in mitigating biotic and abiotic stress.This book will be helpful for students, teachers, researchers, and entrepreneurs involved in PGPR and allied fields. The book will be highly useful to researchers, teachers, students, entrepreneurs, and policymakers.

Plant tissue culture and advanced biotechnologies have proven to be influential tools that complement conventional breeding and accelerate development of many medicinal plants. Various approaches, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors and scaling up in bioreactors, have been explored to improve the production of secondary metabolites from different medicinal plants. This book provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of Catharanthus roseus, Silybum marianum and Digitalis species which have been considered as alternative sources for the production of anti-tumour compounds, flavonolignans and cardenolides. Specific focus is on elicitation strategy for increasing production of bioactive compounds of C. roseus L., S. marianum and Digitalis species to overcome the constrains of conventional propagation. This book is valuable for researchers or students working on medicinal plants, phytochemistry, and plant tissue culture. It also serves as a reference for the pharmaceutical industry.

This book presents the latest information on the genetics and genomics of the globe artichoke. It focuses on the latest findings, tools and strategies employed in genome sequencing, physical map development and QTL analyses, as well as genomic resources. The re-sequencing of four globe artichoke genotypes, representative of the core varietal types in cultivation, as well as the genotype of cultivated cardoon, has recently been completed. Here, the five genomes are reconstructed at the chromosome scale and annotated. Moreover, functional SNP analyses highlight numerous genetic variants, which represent key tools for dissecting the path from sequence variation to phenotype, as well as for designing effective diagnostic markers. The wealth of information provided here offers a valuable asset for scientists, plant breeders and students alike.

Increasing agro productivity to feed a growing global population under the present climate scenario requires optimizing the use of resources and adopting sustainable agricultural production. This can be achieved by using plant beneficial bacteria, i.e., those bacteria that enhance plant growth under abiotic stress conditions, and more specifically, microorganisms such as plant growth promoting rhizobacteria (PGPR), which are the most promising candidates in this regard. Attaining sustainable agricultural production while preserving environmental quality, agro-ecosystem functions and biodiversity represents a major challenge for current agricultural practices; further, the traditional use of chemical inputs (fertilizers, pesticides, nutrients etc.) poses serious threats to crop productivity, soil fertility and the nutritional value of farm produce. Given these risks, managing pests and diseases, maintaining agro-ecosystem health, and avoiding health issues for humans and animals have now become key priorities. The use of PGPR as biofertilizers, plant growth promoters, biopesticides, and soil and plant health managers has attracted considerable attention among researchers, agriculturists, farmers, policymakers and consumers alike. Using PGPR can help meet the expected demand for global agricultural productivity to feed the world's booming population, which is predicted to reach roughly 9 billion by 2050. However, to do so, PGPR strains must be safe for the environment, offer considerable plant growth promotion and biocontrol potential, be compatible with useful soil rhizobacteria, and be able to withstand various biotic and abiotic stresses. Accordingly, the book also highlights the need for better strains of PGPR to complement increasing agro-productivity.

This book presents a comprehensive overview of plant stresses caused by salt, drought, extreme temperatures, oxygen and toxic compounds, which are responsible for huge losses in crop yields. It discusses the latest research on the impact of salinity and global environment changes, and examines the advances in the identification and characterization of the mechanisms that allow plants to tolerate biotic and abiotic stresses. Further it presents our current understanding of metabolic fluxes and the various transporters that collectively open the possibility of applying in vitro technology and genetic engineering to improve stress tolerance. Exploring advanced methods that augment traditional plant tissue culture and breeding techniques toward the development of new crop varieties that can tolerate biotic and abiotic stresses to achieve sustainable food production, this book is a valuable resource for plant scientists and researchers.

This book provides in-depth insights into the regulatory frameworks of five countries and the EU concerning the regulation of genome edited plants. The country reports form the basis for a comparative analysis of the various national regulations governing genetically modified organisms (GMOs) in general and genome edited plants in particular, as well as the underlying regulatory approaches.The reports, which focus on the regulatory status quo of genome edited plants in Argentina, Australia, Canada, the EU, Japan and the USA, were written by distinguished experts following a uniform structure. On this basis, the legal frameworks are compared in order to foster a rational assessment of which approaches could be drawn upon to adjust, or to completely realign, the current EU regime for GMOs. In addition, a separate chapter identifies potential best practices for the regulation of plants derived from genome editing.

This book highlights the advances in essential oil research, from the plant physiology perspective to large-scale production, including bioanalytical methods and industrial applications. The book is divided into 4 sections. The first one is focused on essential oil composition and why plants produce these compounds that have been used by humans since ancient times. Part 2 presents an update on the use of essential oils in various areas, including food and pharma industries as well as agriculture. In part 3 readers will find new trends in bioanalytical methods. Lastly, part 4 presents a number of approaches to increase essential oil production, such as in vitro and hairy root culture, metabolic engineering and biotechnology. Altogether, this volume offers a comprehensive look at what researchers have been doing over the last years to better understand these compounds and how to explore them for the benefit of the society.

With the recent shift of chemical fertilizers and pesticides to organic agriculture, the employment of microbes that perform significant beneficial functions for plants has been highlighted. This book presents timely discussion and coverage on the use of microbial formulations, which range from powdered or charcoal-based to solution and secondary metabolite-based bioformulations. Bioformulation development of biofertilizers and biopesticides coupled with the advantages of nanobiotechnology propose significant applications in the agricultural section including nanobiosensors, nanoherbicides, and smart transport systems for the regulated release of agrochemical. Moreover, the formulation of secondary metabolites against individual phytopathogens could be used irrespective of geographical positions with higher disease incidences. The prospective advantages and uses of nanobiotechnology generate tremendous interest, as it could augment production of agricultural produce while being cost-effective both energetically and economically. This bioformulation approach is incomparable to existing technology, as the bioformulation would explicitly target the particular pathogen without harming the natural microbiome of the ecosystem. Nanobiotechnology in Bioformulations covers the constraints associated with large-scale development and commercialization of bioinoculant formations. Furthermore, exclusive emphasis is be placed on next-generation efficient bioinoculants having secondary metabolite formulations with longer shelf life and advanced competence against several phytopathogens. Valuable chapters deal with bioformulation strategies that use divergent groups of the microbiome and include detailed diagrammatic and pictorial representation. This book will be highly beneficial for both experts and novices in the fields of microbial bioformulation, nanotechnology, and nano-microbiotechnology. It discusses the prevailing status and applications available for microbial researchers and scientists, agronomists, students, environmentalists, agriculturists, and agribusiness professionals, as well as to anyone devoted to sustaining the ecosystem.

Sugarcane exhibits all the major characteristics of a promising bioenergy crop including high biomass yield, C4 photosynthetic system, perennial nature, and ratooning ability. Being the largest agricultural commodity of the world with respect to total production, sugarcane biomass is abundantly available. Brazil has already become a sugarcane biofuels centered economy while Thailand, Colombia, and South Africa are also significantly exploiting this energy source. Other major cane producers include India, China, Pakistan, Mexico, Australia, Indonesia, and the United States. It has been projected that sugarcane biofuels will be playing extremely important role in world's energy matrix in recent future. This book analyzes the significance, applications, achievements, and future avenues of biofuels and bioenergy production from sugarcane, in top cane growing countries around the globe. Moreover, we also evaluate the barriers and areas of improvement for targeting efficient, sustainable, and cost-effective biofuels from sugarcane to meet the world's energy needs and combat the climate change.

This book presents recent advances in global wheat crop research, including the effects of abiotic stresses like high and low temperatures, drought, hypoxia, salinity, heavy metals, nutrient deficiency, and toxicity on wheat production. It also highlights various approaches to alleviate the damaging effects of abiotic stress on wheat as well as advanced approaches to develop abiotic-stress-tolerant wheat crops. Wheat is probably one of the world's most important cereals; it is a staple food in more than 40 countries, and because of its adaptability is cultivated in almost every region. Global wheat production has more than doubled in the last 50 years due to higher yields. However, despite their high yield potential, modern wheat cultivars are often subject to crop loss due to the abiotic stresses. As such, plant breeders have long aimed to improve tolerance in order to maintain yield. Written by 85 experts, and offering the latest insights into wheat responses and tolerance to various abiotic stresses, it is a valuable tool for agronomists, plant breeders, plant physiologists and students in the field of plant science and agriculture. It is the first book to comprehensively cover past and current abiotic stress problems and tolerance mechanisms.