Advancing Development of Synthetic Gene Regulators - With the Power of High-Throughput Sequencing in Chemical Biology (Hardcover, 1st ed. 2018)

This book focuses on an "outside the box" notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)-N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.

General

Imprint:	Springer Verlag, Singapore
Country of origin:	Singapore
Series:	Springer Theses
Release date:	September 2017
First published:	2018
Authors:	Anandhakumar Chandran
Dimensions:	235 x 155mm (L x W)
Format:	Hardcover
Pages:	114
Edition:	1st ed. 2018
ISBN-13:	978-981-10-6546-0
Categories:	Books > Medicine > Clinical & internal medicine > Gene therapy Books > Science & Mathematics > Biology, life sciences > Biochemistry > General Books > Professional & Technical > Biochemical engineering > Biotechnology > General
LSN:	981-10-6546-2
Barcode:	9789811065460

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