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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical)
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R-Loops - Methods and Protocols (Hardcover, 1st ed. 2022)
Loot Price: R6,342
Discovery Miles 63 420
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R-Loops - Methods and Protocols (Hardcover, 1st ed. 2022)
Series: Methods in Molecular Biology, 2528
Expected to ship within 12 - 19 working days
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This detailed book compiles a series of laboratory protocols
covering the most important aspects of R-loop biology. Beginning
with a range of methods allowing for the detection of DNA-RNA
hybrids, as well as their purification and visualization by
electron microscopy, the volume continues with methods based on the
use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and
in vivo. Several protocols permit studying non-canonical RNA
nucleotides in the R-loop context, as well as a number of specific
protocols devoted to the investigation of R-loop topology and their
functional roles in the biology of mitochondria and telomeres.
Finally, a large block of chapters is dedicated to different
methods allowing genome-wide mapping of DNA-RNA hybrids in various
organisms. Written for the highly successful Methods in Molecular
Biology series, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, R-Loops: Methods and Protocols serves as an ideal
resource for those working on R-loop homeostasis but also to
scientists studying such areas of molecular and cell biology as
genome integrity, DNA replication and repair, chromatin remodeling,
transcription, RNA processing, modification and export, as well as
for researchers elucidating the molecular mechanisms of cancer and
genetic diseases.
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