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Books > Professional & Technical > Mechanical engineering & materials > Production engineering
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Recombinant Enzymes - From Basic Science to Commercialization (Hardcover, 2015 ed.)
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Recombinant Enzymes - From Basic Science to Commercialization (Hardcover, 2015 ed.)
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This edited work presents studies that clarify the basics of
producing recombinant enzymes that finally lead to
commercialization. It enables researchers to see what is crucial to
the commercialization process, from examining the cloning method,
using analytical techniques such as calculating the total protein
content and enzyme activity, through considering upstream and
downstream processes, to the final product. Readers will discover
the importance of the cloning method as it influences the upstream
and downstream processes and determines the level of success of the
recombinant enzyme commercialization processes. We see that the two
main factors that are particularly sensitive during the cloning
process are the vector and the host. A discussion of analytical
techniques is presented followed by studies on important stages
during the upstream processes including the process of optimizing
the media to get results and high enzyme activity. Downstream
processes such as the cell disruption technique, purification and
formulation of the final product are then considered. The reader is
introduced to software that helps streamline recombinant enzyme
production from the upstream to downstream processes, to facilitate
the process of up-scaling production. This work includes a case
study as tool, to guide understanding of the commercialization
process. The work is written for researchers in the field and is
especially suited to those who are under pressure to embark on the
tough process of commercialization.
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