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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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RNAi and microRNA-Mediated Gene Regulation in Stem Cells - Methods, Protocols, and Applications (Hardcover, 2010)
Loot Price: R4,282
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RNAi and microRNA-Mediated Gene Regulation in Stem Cells - Methods, Protocols, and Applications (Hardcover, 2010)
Series: Methods in Molecular Biology, 650
Expected to ship within 12 - 17 working days
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Recent stem cell research has revealed that miRNA and RNAi-mediated
gene regulation is one of the vital determinates controlling the
state of cell differentiation, with the small RNAs serving as key
elements involved in regulatory network control of pluripotent cell
fate determination. In RNAi and microRNA-Mediated Gene Regulation
in Stem Cells: Methods, Protocols, and Applications, expert authors
from laboratories across the globe contribute an accessible
compendium of up-to-date, proven methods focused on the study of
the titular topic. Divided into three sections, the book first
gives a brief introduction to RNAi and miRNAs in stem cells, with a
focus on the current status of research and future perspectives,
then it continues with detailed methods and protocols for RNAi
screening, transfection, and the knockdown of specific genes and
pathways in several animal species, including humans and mice,
concluding with a section on recently developed methods for
identification of miRNAs, including a general protocol for
preparation and analysis of miRNA libraries for deep sequencing,
knock down of a specific gene using miRNA-based shRNA, and miRNA
expression analysis using qRT-PCR. Written in the highly successful
Methods in Molecular Biology (TM) series format, chapters contain
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes highlighting tips on
troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem
Cells: Methods, Protocols, and Applications serves as a valuable
resource for scientists and aspiring graduate students interested
in the intersection of RNAi, miRNA, and stem cell molecular biology
and the exciting areas of medicine, including regenerative
medicine, aging, cancer, and neurological disorders, that can be
advanced through this expanding area of research.
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