Development of a Fully Integrated "Sample-In-Answer-Out" System for Automatic Genetic Analysis (Hardcover, 1st ed. 2018)

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice.

General

Imprint:	Springer Verlag, Singapore
Country of origin:	Singapore
Series:	Springer Theses
Release date:	July 2017
First published:	2018
Authors:	Bin Zhuang
Dimensions:	235 x 155mm (L x W)
Format:	Hardcover
Pages:	114
Edition:	1st ed. 2018
ISBN-13:	978-981-10-4752-7
Categories:	Books > Medicine > Nursing & ancillary services > Biomedical engineering > General Books > Science & Mathematics > Biology, life sciences > Biochemistry > General Books > Professional & Technical > Biochemical engineering > Biotechnology > Genetic engineering
LSN:	981-10-4752-9
Barcode:	9789811047527

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