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Books > Professional & Technical > Biochemical engineering > Biotechnology > Genetic engineering
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Gene Transfer and Expression Protocols (Hardcover)
Loot Price: R4,463
Discovery Miles 44 630
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Gene Transfer and Expression Protocols (Hardcover)
Series: Methods in Molecular Biology, 7
Expected to ship within 10 - 15 working days
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Murray's new handbook on Gene Transfer and Expression Protocols
sets forth both current and new methodologies in a clear, concise,
easy-to-follow manner, following the successful formula of the
classic volumes in Humana's Methods in Molecular Biology series.
Each chapter is devoted to a thorough exposition of a single
technique. An Introduction explains the significance of the
protocol and provides background information. A Materials section
lists all the requirements for the technique discussed. A Methods
section details the procedure in a step-by-step protocol. A Notes
section alerts the reader to pitfalls that may be encountered, as
well as alternatives that may be used for successful completion of
the experiment. Each technique is designed to guarantee optimum
results.
This volume is an outstanding new benchtop manual that provides
protocols for introducing an isolated gene into a cell line, using
various transfection techniques. Topics and techniques include:
viral vectors a [ reporter genes a [ analysis of steady-state
level of transcription a [ assay for newly initiated
transcriptional complexes a [ immunocytological techniques a [
assay of structure and replication state of transfected genes a [
overall strategy for defining regulatory sequences
Providing the latest techniques in a fast-paced area, Gene
Transfer and Expression Protocols is an absolutely essential
handbook for everyone involved in cytogenetics, cell genetics,
molecular biology, and related fields.
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