In the last decade, a large number of major discoveries have shed
light on the molecular mechanisms of lymphocyte migration and the
anatomy of immune responses. In T-Cell Trafficking: Methods and
Protocols, expert researchers explore how the development of novel
and cutting-edge techniques, particularly in the field of real-time
imaging and genetic manipulation, have led to an increased
understanding of lymphocyte trafficking. Written by internationally
recognized experts in their respective fields, chapters provide
state-of-the-art protocols to study lymphocyte migration and
T-cell: endothelial cell interactions in vitro, address various
approaches used for direct visualization of the development of the
lymphoid system, lymphocyte recirculation, and effector responses
in experimental models in vivo, and explore lymphocyte migration
and inflammation in the human system. Composed in the highly
successful Methods in Molecular Biology (TM) series format, each
chapter contains a brief introduction, step-by-step methods, a list
of necessary materials, and a Notes section which shares tips on
troubleshooting and avoiding known pitfalls. Innovative and highly
practical, T-Cell Trafficking: Methods and Protocols is an
essential manual for newcomers in this ever-expanding and exciting
area of research, as well as a valuable addition to more
specialized laboratories.
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