The imaging of small cellular components requires powerful
instruments, and an entire family of equipment and techniques based
on the confocal principle has been developed over the past 30
years. Such methods are commonly used by neuroscience researchers,
but the majority of these users do not have a microscopy or a cell
biology backgrounds and do can encounter difficulties in obtaining
and interpreting results. This volume brings experts in
high-resolution optical microscopy applications in neuroscience and
cell biology together to document the state of the art. Outlining
what is currently possible, the volume also discusses promising
developments for the future and aids readers in selecting the most
scientifically meaningful approach to solve their questions. Each
chapter discusses instrumentation and technology in relationship to
application in research. All of the common and cutting edge trends
are covered - fluorescence / laser electron / nonlinear microscopy,
infrared fluorescence, multiphoton imaging, tomography, FRAP, live
imaging, STED, PALM/STORM, etc.
* Single and multiphoton confocal microscopy, and 4-pi confocal
* Obtaining nanoresolution via photoactivation localization
* Several procedures that correlate observations in optical
fluorescence microscopy and electron microscopy
* Study of morphology and function via high-resolution fluorescence
* Additional high-resolution microscopic techniques
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