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Books > Professional & Technical > Biochemical engineering > Biotechnology
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Methods in Gut Microbial Ecology for Ruminants (Hardcover, 2005 ed.)
Loot Price: R6,009
Discovery Miles 60 090
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Methods in Gut Microbial Ecology for Ruminants (Hardcover, 2005 ed.)
Expected to ship within 10 - 15 working days
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Until recently, knowledge of ruminant gut microbiology was
primarily obtained using classical culture based techniques, which
probably only account for 10 to 20% of the rumen microbial
population. New gene-based technologies can now be employed to
examine microbial diversity through the use of small sub-unit
ribosomal DNA analysis (e.g. 16S rDNA) and to understand the
function of complex microbial ecosystems in the rumen through
metagenomic analysis. These technologies have the potential to
revolutionize the understanding of rumen function and will overcome
the limitations of classical based techniques, including isolation
and taxonomic identification of strains important to efficient
rumen function and better understanding of the roles of
microorganisms in relation to achieving high productivity and
decreasing environmental pollutants. This book presents a
comprehensive up-to-date account of the methodologies and protocols
for conventional and modern molecular techniques that are currently
in use for studying the gut microbial ecology of ruminants. Each
chapter has been contributed by experts in the field and methods
have been presented in a recipe-like format designed for direct
practical use in the laboratory and also to provide insight into
the most appropriate techniques, their applications and the type of
information that could be expected. The techniques and procedures
described are also relevant and adaptable to other gastrointestinal
ecosystems and the microbiology of anaerobic environments in
general. This manual will a ~demystifya (TM) the methods in
molecular microbial ecology for readers who are novice in the field
but are excited by the prospects of the technology. Itwould also be
invaluable for the experienced workers striving for giving new
dimension to their research a" expanding the work in other fields
and initiating cross-cutting activities.
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