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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Identification and Expression of G-Protein Coupled Receptors (Paperback)
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Identification and Expression of G-Protein Coupled Receptors (Paperback)
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Identification and Expression of G Protein-Coupled Receptors edited
by Kevin R. Lynch The past decade has seen tremendous advances in
the study of G protein-coupled receptors (GPCRs), including the
molecular cloning and identification of more than 100 GPCR genes.
But while GPCRs serve as targets for more than 300 medicines in the
modern pharmacopoeia, the shrinking pool of known ligands and the
continuing discovery of orphan GPCR genes have underscored the need
for new approaches to ligand identification. Identification and
Expression of G Protein-Coupled Receptors addresses this new
direction in GPCR biochemistry-offering a definitive laboratory
bench manual that emphasizes expression over primary cloning
strategies. In a series of expert contributions by well-known
researchers, this book provides detailed protocols for various
expression systems-from bacteria to mammalian cells-as well as
straightforward opinions on the advantages and shortcomings of each
approach. Topics covered include:
* Homology screening and the polymerase chain reaction in the
cloning of GPCR genes
* Cloning of GPCRs using mammalian cell expression
* GPCR informatics and the orphan problem
* The use of Xenopus laevis oocytes for the study of GPCRs
* Stable expression of GPCRs in mammalian cells
* Heterologous expression in primary cell cultures
* Expression of GPCRs in Escherichia coli
* Large scale expression and purification of GPCRs in mammalian
cells
* High-level expression of GPCRs in the Baculovirus/Sf9 cell
expression system
* Expression of GPCRs in Drosophila Schneider 2 cells
* Methods for genetic analysis and ligand identification using
heterologous GPCRsexpressed in Saccharomyces cerevisiae
Supplemented with numerous photographs and illustrations,
Identification and Expression of G Protein-Coupled Receptors is
important reading for biochemists, pharmacologists,
neuroscientists, structural biologists, and anyone involved in
GPCR-based research. It delivers a wealth of useful advice,
practical tips, and invaluable insight into trends at the
cutting-edge of current research. Also in this series: Receptor
Localization: Laboratory Methods and Procedures edited by Marjorie
A. Ariano
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